采用雌性SD大鼠探索建立适宜于气滞血瘀证妇科疾病特点的模型评价体系  

Study on evaluation system for gynecological disease model characterized by Qi stagnation and blood stasis syndrome with female SD rats

作　　者：李炜[1] 段金廒[1] 刘培[1] 史旭芹[1] 李振皓[1] 范欣生[1] 华永庆[1] 郭建明[1] 

机构地区：[1]南京中医药大学江苏省方剂高技术研究重点实验室,江苏南京210046

出　　处：《中国中药杂志》2013年第8期1211-1218,共8页China Journal of Chinese Materia Medica

基　　金：江苏省高校自然科学重大基础研究项目(06KJA36022);江苏高校优势学科建设工程项目(ysxk-2010);江苏省自然科学基金项目(BK2012456)

摘　　要：目的:建立适宜于气滞血瘀证妇科疾病特点的动物模型评价体系,以临床经典方反证模型证候特点,从宏观生物体征评价模型专属性及可靠性。方法:选用SD雌性大鼠,采用2周皮下注射盐酸肾上腺素及随机昼夜颠倒、冰水游泳、烘箱热烘、噪音刺激、悬尾慢性不可预见性刺激复制模型,灌胃香附四物汤、少腹逐瘀汤及阳性对照药阿司匹林,以血液流变学、凝血4项指标、四诊信息(爪、尾、舌数码拍照;体温检测;体重;摄食量;心电图采集;旷场自主活动)、证候积分综合评价模型。结果:与正常组相比,模型组在血液流变学、凝血4项指标、动物行为学、体重、饮食量、证候积分、心率方面均有明显变化;拍摄舌象、爪像经Photoshop 7.0分析所得大红色(r)、绿色(g)、蓝色(b)的百分含量与正常组相比有显著性差异;阿司匹林及香附四物汤给药组在血液流变学、凝血4项、动物行为学、体重、饮食量、心率、证候积分及舌、尾r,g,b有显著改善作用,而少腹逐瘀汤未见明显改善作用。结论:本文建立了适宜于气滞血瘀证妇科疾病特点的动物模型评价体系,为气滞血瘀证模型评价体系研究提供借鉴。Objective ： To establish an evaluation system for animal model with gynecological disease characterized by Qi stag- nation and blood stasis syndrome, in order to disprove syndrome characteristics of the model by classic clinical prescriptions, and eval- uate the specificity and reliability of the model with macroscopic biological signs and symptoms. Method ： The model characterized by Qi stagnation and blood stasis syndrome was established by injecting adrenaline into female SD rats and conducting unpredictable chron- ic stimulus such as reversal of day and night, swimming in cold water, thermal stimulation in oven, noise and tail suspension for two weeks. They were also orally administered with Xiangfu Siwu Tang, Shaofu Zhuyu Tang and positive control drug aspirin in groups. A comprehensive evaluation was conducted for the model on the basis of haemorheology, four blood coagulation indexes, four diagnostic information （ digital imaging of tongue, paw and tail, temperature, weight, ingestion, electrocardiograph, and open filed test）, and syndrome rating. Result： Compared with the normal group, the model group showed obvious changes in haemorheology, four blood co- agulation indexes, animal behavior, weight, ingestion, syndrome rating and heart rate. Their tongue and paw pictures were analyzed with Photoshop 7. 0, showing significant difference in red, green and blue percentage composition from the normal group. Groups given aspirin and Xiangfu Siwu Tang showed notable changes in haemorheology, four blood coagulation indexes, animal behavior, weight, in- gestion, heart rate, syndrome rating, and red, green and blue percentage composition in tongue and paw pictures, whereas the group given Shaofu Zhuyu Tang showed no remarkable improvement. Conclusion： The evaluation system for the animal model with gynecolog- ical disease characterized by Qi stagnation and blood stasis syndrome is established to provide reference for studies on the evaluation system for qi stagnation and blood stasis syndrome models.

关 键 词：气滞血瘀证 证候模型 香附四物汤 少腹逐瘀汤 

分 类 号：R271[医药卫生—中医妇科学]