醛糖还原酶5’端两种基因多态性与2型糖尿病肾病的相关性研究  

The Relationship between the Two Polymorphisms at the 5’-end of the Aldose Reductase Gene and Diabetic Nephropathy

作　　者：李艳[1] 刘长山[2] 程硕[1] 

机构地区：[1]潍坊医学院,山东潍坊261041 [2]潍坊市人民医院,山东潍坊261041

出　　处：《中国医药指南》2013年第6期5-6,共2页Guide of China Medicine

摘　　要：目的探讨醛糖还原酶（AR）基因启动子区C（-106）T单核苷酸多态性和2.1Kb处（AC）n二核苷酸微卫星多态性与2型糖尿病肾病（DN）的关系。方法用聚合酶链反应（PCR）、测序及琼脂糖凝胶电泳技术检测AR基因两多态位点的基因型及等位基因,比较各组分布频率。结果在DM组和CON组均发现AR基因C（-106）T多态位点存在C、T两种等位基因和CC、CT、TT三种基因型,（AC）n序列的7种等位基因（Z-6~Z＋6）;T、Z-2等位基因及CT＋TT及含有Z-2的基因型在DN组明显高于NDN和CON组,但在DM组和CON组之间无显著性差异。结论 AR基因C（-106）T多态位点的T等位基因及（AC）n多态位点的Z-2等位基因可能是DN的易感基因。Objective To investigate the relationship of aldose reductase gene promoter area C（-106）T single nucleotide polymorphism and 2.1 Kb place（AC）n dinucleotide microsatellite polymorphism and type 2 diabetes nephropathy.Methods Using polymerase chain reaction（PCR）,sequencing and agarose gel electrophoresis technology detection AR gene genotype and allele,compare the frequency distribution.Result T,Z-2 allele and CT＋TT,Z-2 genotype in DN group is obviously higher than that of the NDN and CON group,but in DM group and CON no significant differences.Conclusion Both the T allele of C（106）T polymorphism and the Z-2 allele of（AC）n polymorphism at AR gene may be susceptibility genes of DN.

关 键 词：2型糖尿病 醛糖还原酶 糖尿病肾病 遗传 

分 类 号：R587.2[医药卫生—内分泌]