一种席夫碱钴(Ⅱ)配合物的合成及其与DNA的相互作用  

Synthesis of a Schiff base cobalt(Ⅱ) complex and its interaction with DNA

作　　者：丁应涛[1] 王立衡[1] 郑成凤[1] 蒋淑恋 黄立漳 高飞[1] 汪庆祥[1] 

机构地区：[1]漳州师范学院化学与环境科学系,福建漳州363000 [2]漳州市产品质量监督检验所,福建漳州363000

出　　处：《化学试剂》2013年第4期313-317,352,共6页Chemical Reagents

基　　金：国家自然科学基金资助项目(21275127;2080-5041);国家质检总局科技计划项目(2011QK217);福建省自然科学基金资助项目(2011J01059);福建省教育厅重点项目(JA11166);福建省高校杰出青年科研人才计划资助项目(JA10201)

摘　　要：以邻苯二胺、水杨醛和乙酸钴为原料,合成了一种新型的席夫碱钴(Ⅱ)配合物(CoL,L=双水杨醛缩邻苯二胺),采用红外光谱和元素分析对配体及配合物结构进行了表征。在pH 7.0的Britton-Robinson(B-R)缓冲溶液中,采用紫外光谱法、粘度法和循环伏安法研究了该席夫碱钴(Ⅱ)配合物与DNA的相互作用。紫外光谱实验表明DNA的加入能引起配合物特征吸收峰的减色效应,表明了二者之间的相互作用,通过光谱滴定法测得二者的结合常数为1.1×104L/mol。粘度实验显示,随着配合物浓度的加大,DNA相对粘度先增大后减小,说明二者之间通过部分嵌插模式结合。电化学实验表明配合物在玻碳电极上有一对对应于中心Co(Ⅱ)离子的氧化还原峰,当加入一定量的DNA后,该对氧化还原峰电流显著降低,进一步表明席夫碱钴配合物与DNA发生了相互作用。A novel Schiff base cobalt（Ⅱ） complex（CoL,L=bissalicyladimine） was synthesized using o-phenylenediamine,salicylaldehyde and cobalt（Ⅱ） acetate as source,and its structure was characterized by IR spectroscopy and elemental analysis.In pH 7.0 Britton-Robinson（B-R） buffer solution,the interaction of the cobalt complex with DNA was investigated by UV spectroscopy,viscosity and cyclic voltammetry.UV spectrum showed that the characteristic absorption peaks of the CoL complex was hypochromic effect when DNA was added,suggesting the interaction of the two species.The binding constant of the two species was determined to be 1.1×104 L/mol through spectroscopic titration method.Viscosity experiments revealed that the cobalt complex bound to DNA via partial intercalation.The electrochemistry of the complex on a glassy carbon electrode showed a pair of redox peaks corresponding to the electron transfer of center cobalt ion.After interaction with DNA,the peak current of the complex decreased,confirming the interaction between the CoL complex and DNA.

关 键 词：席夫碱 钴(Ⅱ)配合物 DNA结合 部分嵌插 

分 类 号：O625[理学—有机化学]