当药组织培养及无性系建立的研究  

The Research on Tissue Culture and Establishing the Clone of Swertia pseudochinensis

作　　者：李茜茹[1] 周清[1] 荆婷[1] 杨文新[1] 姜长阳[1] 

机构地区：[1]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《中国园艺文摘》2013年第3期9-10,8,共3页Chinese Horticulture Abstracts

基　　金：辽宁省普通高等教育本科教学改革研究项目(201203041-4);辽宁省大学生创新创业训练项目(201203015011)

摘　　要：为保护资源和实现栽培,以当药的嫩茎为材料,进行愈伤组织诱导与分化、不定芽生根和试管苗生根继代增殖培养研究,建立起当药的嫩茎无性系。结果证明:MS+ZT0.3mg/L+2,4-D2.1mg/L是诱导愈伤组织的理想培养基;MS+AgNO30.5mg/L+BA0.2mg/L+KT0.4mg/L+NAA0.2mg/L是愈伤组织分化培养的理想培养基;向分化不定芽的培养瓶中倒入3～5ml浓度为0.5mg/L的NAA溶液对不定芽处理24h,接种到White+ABT2号0.5mg/L培养基上进行生根培养的方法是不定芽生根培养的理想方法;White+ABT2号0.5mg/L+NAA0.5mg/L是试管苗继代增殖培养的理想培养基。试管苗移栽成活率为94.0%～95.2%,定植成活率为97.3%。定植的试管苗当年开花结果,并保持野生当药的植物学性状。In order to preserve the resources and meet the needs of planting, tender stems of Swertia pseudochinensis were cultured in vitro to nvestigate callus inducement and differentiation, formation of adventitious roots, rooting multiplication of tube seedlings and the clones of Swertia pseudochinensis from tender stems was established. The results indicated that： the ideal medium for callus inducement was MS＋ZT 0.3 mg/L＋2,4-D 2.1 mg/L; MS＋AgNO3 0.5 mg/L＋BA 0.2 mg/L＋KT 0.4 mg/L＋NAA 0.2 mg/L was suitable for callus differentiation; cultivated in the medium White＋NO.ABT2 0.5 mg/L after the adventitious buds disposed 24 hours with 3 ～ 5 ml NAA 0.5 mg/L was the best method for rooting of adventitious buds; White＋ABT2 NO.0.5 mg/L＋NAA 0.5 mg/L was the best medium for rooting multiplication of tube seedlings. The transplanting survival rate of tube seedlings was 94.0% ～ 95.2% and stable planting survival rate was 97.3%, the tube seedlings transplanted beared the following year and maintained all botany characteristics of the wild Swertia pseudochinensis.

关 键 词：当药 组织培养 无性系 

分 类 号：S567.239[农业科学—中草药栽培]