大承气汤及其不同部位治疗内毒素血症的药效机理研究  

Study on Pharmcodynamic Mechanism of Dachengqi Decoction and Its Different Positions Treating Endotoxemia

作　　者：耿耘[1] 彭春荣[1] 马超英[1] 杨超[1] 涂显琴[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《中华中医药学刊》2013年第4期748-750,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30873365);中央高校基本科研业务费专项研究资助项目(SWJTU09ZT29)

摘　　要：目的:探讨大承气汤及其不同部位对于内毒素血症的治疗作用;方法:将105只雄性昆明小鼠随机分为空白组、模型组、大承气汤组、氯仿组、乙酸乙酯组、乙醇组和醇沉组,每组15只。空白组腹腔消毒注射生理盐水(10 mg/kg),其余各组腹腔消毒注射LPS(10 mg/kg)。治疗组于攻毒前0.5 h、攻毒后1 h和取血前0.5 h给予药液8.11 g/(kg.d)灌胃;空白组和模型组于攻毒前0.5 h、攻毒后1 h和取血前0.5 h按比例给与生理盐水。8h后在无菌条件下采集心室血0.5 mL进行血浆ET检测,取部分肝脏进行免疫组化检测,取心、肝、肾进行组织学观察。结果:大承气汤组和乙酸乙酯组对小鼠的内毒素、TNF-α、IL-1β、IL-10、MDA和心、肝、肾组织病理炎症活动度等改善优于其他治疗组。结论:大承气汤和大承气汤的乙酸乙酯部位对小鼠内毒素血症有显著的疗效,其作用机制可能与抑制炎性细胞因子的释放,清除氧自由基,保护心、肝、肾等多脏器有关。Objective ： To explore the treatment effect of Dachengqi Decoction （DCQD） and its different parts on endo- toxemia. Methods ： 105 male KM mice were divided into 7 groups randomly ： control group, model group, DCQD group, chloroform group, ethyl group ,ethanol group and ethanol precipitation group ,with 15 in each group. Mice of control groupwere abdominal injected NS（ 10 mg/kg）sterile, the others were abdominal injected LPS （10 mg/kg）. The treatment groups were given remedy liquid [ 8.11 g/（ kg · d） ] by 1 g at 0.5 h before and 1 h after attacking and 0.5 h before col- lecting blood;the control group and model group were given NS at the same time. 8 hours later,0.5 mL ventricular blood under sterile conditions was collected and the plasma ET was detected, parts of the liver were collected to do the immuno- histochemistry test, and the heart, liver and kidney were taken out to have the histological observations. Results： The effects of DCQD group and ethyl group were superior to that of other treating groups on improving the endotoxin,TNF - α, IL - 113, IL - 10, MDA and the pathological inflammatory activity of heart, liver and kidney. Conclusions ： The DCQD and its ethyl acetate have obvious effect on endotoxemia. The mechanisms are the followings ： promoting the recovery of gastro- inteztinal function, preventing the bacterial translocation, decreasing the absorption of endotoxin, reducing the excessive in- flammatory response, improving symptoms, stabilizing the balance of the body＇s immune, increasing the blood flow in ab- dominal organs, promoting the absorption and removing inflammatory exudates, scavenging oxygen free radicals, protecting liver sinusoidal endothelial ceils, protecting the heart,liver, kidney and other organ functions and so on.

关 键 词：大承气汤 提取物 内毒素血症 作用机理 

分 类 号：R285.5[医药卫生—中药学]