芍药甘草汤有效组分对慢性坐骨神经结扎大鼠IL-6、IL-1β、TNF-α的影响  

Administration of the Influence of Shaoyao Gancao Decoction Extracts on IL-6 IL-1β and TNF-α in the Chronic Constriction Injury Rat Model of Neuropathic Pain

作　　者：郑冬明[1] 张娟[1] 王锐[1] 吕晨[1] 郭小文[1] 王海娜[2] 

机构地区：[1]浙江省中医院,浙江杭州310006 [2]浙江中医药大学,浙江杭州310053

出　　处：《中华中医药学刊》2013年第4期801-803,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划项目(2009YA005)

摘　　要：目的:观察芍药甘草汤有效组分(SGM)对慢性压迫性损伤(CCI)大鼠机械痛敏和热痛敏的影响,并研究SGM对CCI大鼠脊髓IL-6、IL-1β、TNF-α的影响。方法:所有大鼠用机械缩足反射阈值(MWT)和热缩足潜伏期(TWL)来分别评价大鼠机械痛敏和热痛敏。SD大鼠随机分为5组,即假手术组(Sham组)、CCI组和空白组,生理盐水组(NS组)和芍药甘草汤组(SGM组),各组分别在术前和术后第1、3和7 d分别测定MWT和TWL,手术结束后提取大鼠脊髓后角测定IL-6、IL-1β、TNF-α的水平。结果:和空白组及Sham组相比,CCI组和NS组术后MWT及TWL均明显下降(P<0.01)。SGM组与NS组相比,MWT和TWL在术后1、3和7 d均明显增加(P<0.01)。和空白组和Sham组相比,CCI组和NS组IL-6、IL-1β、TNF-α的水平明显增加(P<0.01)。和NS组比较,SGM组IL-6、IL-1β、TNF-α的水平明显降低(P<0.01)。结论:SGM能抑制CCI大鼠的机械痛敏和热痛敏作用,其镇痛作用可能和减少IL-6、IL-1β、TNF-α的水平有关。Objective：To observe the influence of the extracts of Shaoyao Gancao decoction（SGM） on mechanical allo- dynia and heat hyperalgesia induced by chronic constriction injury （CCT） of the sciatic nerve in rats and study the effect and the changes of IL - 6, IL - 1 β and TNF - α. Methods ： Mechanical allodynia and heat hyperalgesia were evaluated by mechanical withdrawal threshold（MWT） and thermalwithdrawal latency （TWL） respectively. Rats were divided randomly into five groups：NS group,sham group,CCI group,SGM group and control group. As the treatment groups, normal saline （ 2 μL） and SGM （ 120 mg/kg） were orally administered for 7 day after surgery. The TWL and MWT were measured before surgery and on days 1,3 and 7 after surgery. The levels of IL -6 ,IL - 1β and TNF - α in spinal dorsal horn in rats were measured after surgery. Results：Compared to control and sham groups,rats with CCI had significantly decreased PWL and PWT from the first day after surgery to the end of this study（P 〈0.01 ）. SGM group had evidently increased the MWT and TWL（P 〈 0.01 ）. Compared to control and sham groups, rats with CCI had significantly increased IL -6, IL - 1 β and TNF- α（P 〈 0.01 ） ,SGM group had evidently decreased IL- 6, IL- 1 β and TNF -αin comparison to NS group（ P 〈 0.01 ）. Conclusion：SGM oral administeration can evidently increase the MWT and TWL of CCI rats . The decreases of IL - 6, IL - 1 β and TNF - α may be related to the analgesia of SGM.

关 键 词：芍药 甘草 神经病理性疼痛 

分 类 号：R285.5[医药卫生—中药学]