桔梗总皂苷体外抗肺炎支原体作用实验研究  

Study on Anti-Mycoplasma Pneumoniea Activity of Platycodon Saponinsin Vitro

作　　者：张俊威[1] 姚琳[1] 蒙艳丽[1] 许庆瑞[1] 高菲[1] 王伟明[1] 

机构地区：[1]黑龙江省中医研究院,黑龙江哈尔滨150036

出　　处：《中华中医药学刊》2013年第4期868-870,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：哈尔滨市青年科技创新人才研究专项资金资助项目(2012RFQYS041)

摘　　要：目的:通过桔梗总皂苷对肺炎支原体(MP)的MIC、MBC及TIME-KILL曲线测定,观察其在体外对MP生长抑制及杀灭作用。方法:采用微量倍比稀释法测定MIC和MBC,利用定量PCR检测不同孵育时间,不同浓度药物对MP繁殖速度的影响。结果:桔梗总皂苷体外抗MP的MIC为16～64μg.mL-1;MBC为64～128μg.mL-1;桔梗总皂苷浓度为32到64μg.mL-1时,可以降低MP生长速度并呈现一定的量效关系,当浓度达到128μg.mL-1时可完全抑制MP在培养液中的生长繁殖。结果:桔梗总皂苷在体外具抑制MP生长繁殖作用。Objective ： To observe the activity of Platycodon saponins against Mycoplasma Pneumoniea through experi- ments of MIC, MBC and TIME - KILL. Method：MICs and MBCs of platycodon saponins were determined by broth microdi- lution method. MP was incubated in PPLO broth at concentrations equivalent to one, two and four times of the MIC that had previously been determined. The organisms of M. Pneumoniae was incubated at 37℃. The concentrations of MP be- fore and after diferent incubate time were detected by quantitative PCR. Result：MICs of platycodon saponins were 16 -64 μg · mL^- 1 and MBCs were 64 - 128 μg · mL^- 1 against MP in vitro. Replication of MP in PPLO was inhibited by platyc- odon saponins when the concentration was between 32 μg · mL^- 1 and 64μg · mL^- 1. The number of MP in PPLO broth was reduced and the growth was completely inhibited when the concentration of platycodon spaonins reached 128 μg · mL^- 1. Conclusion ：Platycodon saponins has anti -M. Pneumoniea activity in vitro.

关 键 词：桔梗总皂苷 肺炎支原体 最低抑菌浓度 最低杀菌浓度 杀菌时-效曲线 

分 类 号：R375.2[医药卫生—病原生物学]