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作 者:刘卫国[1] 盛雅娟[1] 蓝天[1] 金洪传[2] 毛伟敏[3,4] 王晓稼[3,4]
机构地区:[1]浙江中医药大学,浙江杭州310053 [2]浙江大学医学院附属邵逸夫医院,浙江杭州310003 [3]浙江省肿瘤医院,浙江杭州310022 [4]浙江省中西医结合肿瘤学重点实验室,浙江杭州310003
出 处:《中华中医药学刊》2013年第4期908-910,I0010,共4页Chinese Archives of Traditional Chinese Medicine
摘 要:目的:研究重楼皂苷Ⅱ(PPⅡ)对人乳腺癌细胞的作用及影响机制。方法:MTT法检测PPⅡ对乳腺癌Bcap37、MCF-7、MDA-MB-231、MDA-MB-453细胞增殖抑制作用,流式细胞仪检测PPⅡ对Bcap37和MDA-MB-231细胞周期分布和细胞凋亡的影响,Hoechst 33342染色观察细胞核变化,Western blotting检测蛋白表达。结果:PPⅡ对乳腺癌Bcap37、MCF-7、MDA-MB-231、MDA-MB-453细胞的增殖均具有抑制作用,并呈剂量依赖性;流式分析细胞周期分析PPⅡ阻滞Bcap37细胞在G2/M期,MDA-MB-231细胞在S期和G2/M期,细胞凋亡率呈浓度和时间依赖性;Hoechst33342显示细胞凋亡特征;Western blotting检测凋亡相关蛋白Cleaved-Caspase9和Cleaved-PAPR增多。结论:PPⅡ对乳腺癌细胞系具有增殖抑制作用,其机制可能与引起G2/M期阻滞和促进细胞凋亡有关。Objective : To study the effect of PP Ⅱ on proliferation of human breast cancer cells and its possible influen- cing mechanism. Methods : MTT assay was used to measure the effect of PP Ⅱ on breast cancer cells Bcap37, MCF - 7, MDA -MB -231 and MDA -MB -453 proliferation, the cell cycle and apoptosis were measured by flow cytometry, the Hoechst 33342 was used to observe the nuclei characteristic and Western blotting detection was used for protein expres- sion. Results:PP Ⅱ showd an inhibitory effect on breast cancer Bcap37 ,MCF- 7,MDA- MB -231 and MDA- MB -453 cells and with the dose -dependent. Bcap37 cells treated by PP Ⅱ were blocked in G2/M phase and MDA -MB -231 cells were blocked in S and G2/M phase ; the apoptosis rate appeared time - dose - dependent. Hoechst 33342 staining images showed apoptotic morphology change. Western blotting detection apoptosis related proteins Cleaved - Caspase9 and Cleaved - PAPR were increased. Conclusion : PP Ⅱcan inhibit the growth of breast cancer cells, which is possibly asso- ciated with inducing cell cycle retardation and apoptosis.
分 类 号:R339.23[医药卫生—人体生理学]
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