慢性寒冷应激对高血压大鼠血浆及脑组织中ET-1和CGRP表达的影响  

Influence of Chronic Cold Stress on Expression of ET-1,CGRP in Plasma and Brain Tissue of Renovascular Hypertensive Rats

作　　者：敬开权[1] 曾荣[2] 顾洪丰[3] 陶润知[1] 杨湘怡[1] 聂亚雄[1] 

机构地区：[1]南华大学附属第一医院神经内科,湖南衡阳421001 [2]南华大学附属郴州市第一人民医院神经内科 [3]南华大学生理学教研室

出　　处：《中南医学科学杂志》2013年第2期118-123,共6页Medical Science Journal of Central South China

基　　金：湖南省自然科学基金(08JJ3036)

摘　　要：目的观察慢性寒冷应激对肾血管性高血压大鼠(RHR)血浆及脑组织中内皮素-1(ET-1)和降钙素基因相关肽(CGRP)表达的影响,并探讨内皮素-1(ET-1)和降钙素基因相关肽(CGRP)与高血压脑卒中发生之间的关系。方法将造模成功后的RHR与对照组大鼠(正常血压)各分为慢性寒冷应激组与非应激组,在慢性寒冷应激8周后处死大鼠,ELISA法检测血浆中ET-1和CGRP的表达,免疫组化法检测脑组织中ET-1和CGRP的表达。结果与RHR非应激组比较,RHR应激组大鼠血液及脑组织中ET-1的表达明显升高,CGRP表达明显下降,差异均有显著性(P<0.05);与对照非应激组比较,对照应激组大鼠血液及脑组织中ET-1的表达明显升高,CGRP表达也明显升高,差异均有显著性(P<0.05)。结论慢性寒冷应激能打破RHR体内ET-1与CGRP的平衡,这可能是寒冷应激促使脑卒中发生的病理生理学机制之一。Objective To observe the influence of chronic cold stress on expression of endothelium-1 （ ET-1 ） and calcitonin gene related peptide （CGRP） in plasma and brain tissue of renovaseular hypertensive rats （RHR） ,and to investigate the relationship between ET-1, CGRP and hypertensive stroke. Method The wistar rats were divided into two groups： renovascular hypertensive rats（ RHR） group and control group （normal blood pressure rats）. Each group was further subdivided into two groups： chronic cold stress group and non-chronic cold stress group. At the 8th week of chronic cold stress all subgroups rats were sacrificed,plasma was saved to measure the expression of ET-1 and CGRP by Enzymelinked immunosorbent assay（ELISA） and brain tissue was collected to measure the expression of ET-1 and CGRP by immunohistochemistry. Results In RHR chronic cold stress group, ET-1 was significantly higher while CGRP was significantly lower in plasma and brain tissue compared with the RHR non-chronic cold stress group （P 〈 0.05）. Compared with the control nonchronic cold stress group, ET-1 and CGRP were both significantly higher in plasma and brain tissue of control chronic cold stress group. Conclusion Chronic cold stress can damage the balance of ET-1 and CGRP of HRH,which is possibly related to the pathological damage of cerebrovaseular and hypertensive stroke.

关 键 词：慢性寒冷应激 肾血管性高血压大鼠 内皮素-1 降钙素基因相关肽 脑卒中 

分 类 号：R743.3[医药卫生—神经病学与精神病学]