抗-A和抗-B单克隆抗体对红细胞内血红蛋白释放情况的影响  

The Effect of Anti-A and Anti-B Monoclonal Antibody on Erythrocytic Hemoglobin Release

作　　者：沈木生 张帆 高丽君[3] 秦文斌[3] 

机构地区：[1]鄂尔多斯中心血站,内蒙古鄂尔多斯017010 [2]鄂尔多斯市卫生局干部保健中心 [3]包头医学院血红蛋白研究室

出　　处：《包头医学院学报》2013年第2期1-3,共3页Journal of Baotou Medical College

基　　金：内蒙古教育厅自然科学重点项目(NJ09157)

摘　　要：目的:探讨抗-A、抗-B单克隆抗体对红细胞内血红蛋白电泳释放的影响。方法:用A、B、O、AB 4种血型红细胞与抗-A及抗-B单克隆抗体反应后的产物进行淀粉-琼脂粉混合凝胶电泳,常规电泳后停电30 min再继续电泳,观察血红蛋白的释放情况。结果:A、B、O、AB型红细胞与单克隆抗体反应后血红蛋白释放情况不同,与此同时HbA2的电泳行为也发生变化。结论:单克隆抗体对各型红细胞的血红蛋白释放有影响。Objective： To evaluate the effect of Anti - A and Anti - B monoclonal antibody on erythrocytic hemoglobin elec- trophoresis release. Methods： Reaction products of erythrocytes from blood group A, B, O and AB with their corresponding mon- oclonal antibodies underwent starch - agarose mixed gel electrophoresis, with electrophoresis resumed after a interruption of 30 minutes, and the hemoglobin release from ery throcytes was observed. Results： Erythrocytes in blood group A, B, O and AB changed their hemoglobin release manners upon reacting with their corresponding monoclonal antibodies and the electrophoresis behaviors of HbA2 of these erythrocytes changed as well. Conclusion： Hemoglobin release from erythrocytes is affected by their reaction with monoclonal antibodies. The research established an important approach for the further investigation of the release mechanism.

关 键 词：红细胞 血型 单克隆抗体 血红蛋白释放 

分 类 号：R446.6[医药卫生—诊断学]