紫花苜蓿黄酮的提取与纯化工艺初探  

Extraction and Separation of Alfalfa Flavonoids

作　　者：王成章[1] 文开新[1] 史莹华[1] 严学兵[1] 樊文娜[1] 杜红旗[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《草地学报》2013年第2期365-371,共7页Acta Agrestia Sinica

基　　金：国家牧草产业技术体系(CARS-35);国家"十二五"科技支撑计划项目(2011BAD17804)资助

摘　　要：为探讨紫花苜蓿(Medicago sative L.)黄酮的提取与纯化工艺,采用不同乙醇浓度和不同pH进行提取,然后用不同来源和型号的大孔树脂进行纯化,以得出苜蓿黄酮的最佳提取和纯化方法。结果表明:30%乙醇、水浸提(pH为7.5)对紫花苜蓿黄酮的浸出率最高,分别为0.46%和0.49%,pH在9.0时所得提取物黄酮含量最高为1.61%;pH为2.2～2.4时,对紫花苜蓿黄酮有最好的沉淀效果,所得沉淀物中黄酮含量为9.80%;9种型号的大孔树脂中DS-17对乙醇提取物中紫花苜蓿黄酮有较好的分离效果,所得提取物黄酮为5.87%;在大孔树脂对碱溶酸沉提取物中黄酮的分离中,2组试验均以DM130的效果最好,所得分离物黄酮含量最高分别为24.25%和29.35%。To optimize the process of extracting and separating flavonoids from alfalfa,different methods were adopted to extract and separate the flavonoids systematically.Results showed that 30% ethanol solution and water（pH=7.5） had the best effect on alfalfa flavonoids extraction.Leaching efficiency of the flavonoids were 0.46% and 0.49%,respectively.The highest content of alfalfa flavonoids extracted with water was 1.61% when pH was 9.0.The optimum pH was 2.2 for separation of alfalfa flavonoids and the content of flavonoids in the extractive was 9.80%.Different macroporous resins were used to separate alfalfa flavonoids extracted with ethanol solution.DS-17 showed the best separation effect and the content of flavonoids was 5.87%.The effect of macroporous resins on the separation of alfalfa flavonoids extracted by acid-isolation was analyzed.DM130 had the best effect and the highest contents of alfalfa flavonoids in two treatments were 24.25% and 29.35%,respectively.

关 键 词：紫花苜蓿 黄酮类化合物 提取 纯化 

分 类 号：Q946.889[生物学—植物学]