耦合中空纤维膜超滤分离游离细胞催化合成ATP  

ATP SYNTHESIS BY FREE YEAST CATALYSIS COUPLING HOLLOW FIBER ULTRAFILTRATION SEPARATION

作　　者：段学辉[1] 张嗣良[2] 叶勤[2] 

机构地区：[1]南昌大学,生命科学与食品工程学院,南昌330047 [2]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《微生物学报》2000年第6期633-637,共5页Acta Microbiologica Sinica

基　　金：高等学校骨干教师资助计划资助!( 2 0 0 0 65-0 8)&&

摘　　要：对耦合中空纤维膜超滤分离进行游离细胞催化合成ATP过程进行了实验研究 ,考察了细胞的催化效率和膜组件的操作稳定性。结果显示 ,中空纤维超滤膜的耦合分离能有效地截留反应液中的游离酶 ,其中乙醇脱氢酶 (ADH)和已糖激酶 (HK)的稳态截留效率在 95%以上。耦合膜分离的酵母细胞催化ATP合成反应可重复使用 2 5～ 3 0次 ,酶的利用率比普通分离的细胞提高 2 0～ 2 5倍。中空纤维超滤膜于 0 1Mpa工作压力下连续 1 1批耦合分离操作 ,膜的渗透性无明显下降 ,过滤速率保持在初速率的 95%以上。在稀释速率 0 2 5h- 1 下 ,反应体系保持了连续 5h的ATP高转化率合成与分离耦合的拟稳态操作。A novel process for the ATP production employing free yeast catalysis and coupling a hollow fiber module ultrafiltration separation was studied. The experimental results of biocatalyst half life and operating stability for ultrafiltration showed that the enzymes released from free yeast cell could be cut off efficiently from the product output stream by hollow fiber membrane. The intercepted percentage of ADH and HK reached more than 95% during the steady running. The efficiency of enzymes utilized was 2 0～2 5 fold as much as that of batch reaction. And the time of yeast cells used repeatedly for ATP synthesis reached 2 5～3 0 batches. Under 0 1MPa, the ultrafiltrating speed of hollow fiber membrane could keep on a stabilizing value for 11 batches continuously. The continuous operation mode of ATP synthesis simultaneous product ultrafiltration separation could be maintained in higher conversion rate for 5 0h when the whole system fell in a pseudo stat where the dilution rate was equal to 0 25h -1 .

关 键 词：ATP 中空纤维膜 生物催化合成 耦合膜 

分 类 号：Q814[生物学—生物工程]