周络通胶囊对糖尿病周围神经病变小鼠坐骨神经TGF-β1/Smad3信号通路的影响  

Effects of Zhouluotong Capsules on TGF-β1/Smad3 signaling pathway in diabetic peripheral neuropathy mice

作　　者：张会欣 赵韶华[2] 王宏涛 朱慧明 魏刚 何奇龙 高学东[2] 

机构地区：[1]河北以岭医药研究院,河北省络病重点实验室,国家中医药管理局重点研究室,河北石家庄050035 [2]石家庄以岭药业股份有限公司,河北石家庄050035

出　　处：《中成药》2013年第4期652-655,共4页Chinese Traditional Patent Medicine

基　　金：十二五“重大新药创制”科技重大专项(2011ZX09101-004-02);河北省中医药管理局科研计划项目(2011041)

摘　　要：目的观察周络通胶囊(黄芪、桂枝、当归、生地、细辛等)对糖尿病周围神经病变小鼠坐骨神经TGF-β1/Smad3信号通路的影响。方法 40只KK/Upj-Ay小鼠随机分为模型组、周络通胶囊高、中、低剂量组(6.85、3.43、1.71 g生药/kg),另设10只C57BL/6小鼠对照组。灌胃给药12周,测定运动神经传导速度;荧光定量PCR和West-ern blot法测定坐骨神经转化生长因子-β1(TGF-β1)、受体I(TGF-βRI)、受体II(TGF-βRII)及Smad3表达。结果模型组与对照组比较,运动神经传导速度明显下降(P<0.01),TGF-β1、TGF-βRI、TGF-βRII及Smad3表达明显上升(P<0.01);与模型组比较,周络通组能提高运动神经传导速度(P<0.05,P<0.01),降低TGF-β1、TGF-βRI、TGF-βRII及Smad3表达(P<0.05,P<0.01)。结论周络通胶囊能通过抑制TGF-β1/Smad3信号通路,改善糖尿病周围神经病变小鼠功能。AIM To investigate the effect of Zhouluotong Capsules（Astragali Radix,Cinnamomi Ramulus,Angelicae sinensis Radix,Rehmanniae Radix,Asari Herba,etc.） on TGF-β1/Smad3 signaling pathway in diabetic peripheral neuropathy mice.METHODS Forty KK/Upj-Ay mice were randomly divided into model,Zhouluotong Capsules（6.85,3.43,1.71 g/kg）groups.Ten C57BL/6 mice were selected as control group.Mice were given intragastrically for twelve weeks.Motor nerve conduction velocity was observed.The expressions of transforming growth factor-β1（TGF-β1）,TGF-β1 receptor I（TGF-βRI）,TGF-β1 receptor II（TGF-βRII）,Smad3 were examined by real time PCR and Western blot.RESULTS Compared with the control group,motor nerve conduction velocity deceased and the expressions of TGF-β1,TGF-βRI,TGF-βRII,Smad3 increased in the model group（P0.01）.Motor nerve conduction velocity increased and the expressions of TGF-β1,TGF-βRI,TGF-βRII,Smad3 significantly decreased in Zhouluotong Capsules group（P0.05,P0.01）.CONCLUSION Zhouluotong Capsules have protective effects on diabetic peripheral neuropathy of mice via suppressing TGF-β1/Smad3 signaling pathway.

关 键 词：周络通胶囊 糖尿病周围神经病变 TGF-Β1 TGF-β1受体 SMAD3 

分 类 号：R285.5[医药卫生—中药学]