集胞藻Synechocystissp.PCC6803利用葡萄糖生长与光合能量转化的关系  

Relationship Between the Growth of Synechocystis sp. PCC 6803 on Medium with Glucose and the Photosynthetic Energy Transformation

作　　者：王永红[1] 叶济宇[2] 米华玲[2] 李元广[1] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]中国科学院上海植物生理研究所,上海200032

出　　处：《Acta Botanica Sinica》2000年第11期1122-1125,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金! (2 98760 10 ) ;国家重点基础研究基金! (G19980 10 10 0 ) ;海洋生物技术 !863 (819_Q_0 1) ;生物反应器工程

摘　　要：用PAM叶绿素荧光仪测定了饥饿细胞、光自养细胞和混合营养细胞的叶绿素荧光 ,并对 3种类型细胞的荧光参数 :PSⅡ实际光化学效率 φⅡ和还原型质醌Q-A 进行了比较。用双重转盘磷光机测定了光自养细胞和混合营养细胞的毫秒延迟发光。根据叶绿素荧光动力学分析和毫秒延迟发光的结果及光合电子传递抑制剂 3,4_二氯苯基二甲脲 (DCMU)、二溴百里香醌 (DBMIB)对集胞藻 6 80 3(Synechocystissp .PCC 6 80 3)混合营养生长影响进行了分析 ,集胞藻 6 80 3混合营养培养的生长速率显著高于光自养培养的原因可能在于一是外源葡萄糖没有抑制反而是促进了混合营养细胞的光自养生长 ,二是呼吸基质向质醌库提供电子 ,使光合机构的能量转化加强 ,从而促进了集胞藻6 80 3细胞的利用葡萄糖的合成代谢。Measurements of chlorophyll fluorescence kinetics from dark_starved cells, light_grown cells and mixotrophic cells of Synechocystis sp. PCC 6803 were obtained using a pulse amplitude modulation (PAM) fluorometer. Photosystem Ⅱ photochemical efficiency Ⅱ and the extent of reduction of Q - A in the three kinds of cells described above were compared. The millisecond delayed light emission (MDLE) of light_grown cells and mixotrophic cells were also detected. On the basis of the analysis of fluorescence kinetic parameters, comparison of the slow phase of MDLE and the influence of inhibitors of photosynthetic electron transport 3_(3,4_dichlorophenyl)_1,1_dimethylurea (DCMU), 2,5_dibromo_3_methyl_6_isopropyl_p_benzoquinone (DBMIB) on the mixotrophic growth of Synechocystis sp. PCC 6803, it was concluded that the reasons for higher growth rate under mixotrophic than that under photoautotrophic might be that glucose promoted the photoautotrophic growth of mixotrephic cells and the donation of eletrons to the plastoquinone pool from the respiratory substance and the transform of energy was promoted by photosynthetic system, which provided the energy needed by anabolism of cells caused by the glucose added to the medium.

关 键 词：集胞藻 光合电子传递 葡萄糖 光合能量转化 

分 类 号：Q945.11[生物学—植物学] Q949.2