Revealing the F_actin Networks in Interphase Nuclei of Garlic Clove Cells by Confocal Fluorescence Microscopy  

共聚焦荧光镜检术揭示大蒜鳞片细胞间期核中的F肌动蛋白网络(英文)

作　　者：王冬梅[1] 王学臣[2] 张伟成[3] 

机构地区：[1]河北农业大学农学院,保定071001 [2]中国农业大学生物学院,北京100094 [3]中国科学院上海植物生理研究所,上海200032

出　　处：《Acta Botanica Sinica》2000年第11期1167-1171,共5页Acta Botanica Sinica（植物学报：英文版）

基　　金：TheNationalKeyBasicResearchSpecialFundofChina !(G199990 1170 0 ) ;TheNationalNaturalScienceFoundationofChina !(3 973 0 0 5 0

摘　　要：The interphase nuclei of parenchyma cells and epidermal cells of garlic ( Allium sativum L.) clove were labelled with rabbit anti_actin antibody and FITC_conjugated goat anti_rabbit IgG antibody. The authors observed results with fluorescence microscopy and confocal laser scanning microscopy. The nuclei showed prominent green_yellow fluorescence, indicating the presence of actin in the nuclei. Fluorescence examination with TRITC_phalloidin showed distinctive red fluorescence in the nuclei, indicating that F_actin is present in the nuclei. Confocal laser scanning microscopy indicated the presence of F_actin containing network structures in the nuclei, but the network structures were absent and the nuclei still showed red fluorescence when the cells were treated with cytochalasin D before fixation; the red fluorescence in the nuclei was hard to be observed when the cells were treated with unlabelled phalloidin before the cells were stained with TRITC_phalloidin. These results indicate that F_actin is in the nuclei and forms network structures in the nuclei of garlic cells.采用免疫荧光和荧光分子探针技术与共聚焦激光扫描显微镜观察相结合 ,对大蒜 (AlliumsativumL .)鳞片细胞间期核中是否存在F肌动蛋白进行了研究。结果表明 ,以兔抗肌动蛋白抗体为一抗、FITC_羊抗兔IgG抗体为二抗进行免疫荧光标记实验 ,在荧光镜下观察到蒜瓣薄壁组织的细胞核及表皮细胞核均发出明亮的黄绿色荧光。经共聚焦激光扫描显微镜进一步检查 ,整个细胞核呈黄绿色荧光 ,说明其中含有肌动蛋白。经TRITC_鬼笔环肽(TRITC_phalloidin)标记后 ,细胞核散发出明亮的红色荧光 ,经共聚焦激光扫描显微镜做光切片及三维重组图像 ,发现核中有一个三维网络结构。细胞松弛素D (cytochalasinD)预处理后 ,核中三维网络结构不复存在 ,但核仍显示红色荧光 ;若经非标记phalloidin预处理 ,则核中几乎看不到红色荧光和任何网络残迹。对照实验结果进一步表明 ,F肌动蛋白存在于大蒜细胞核中并以F肌动蛋白纤束形成三维网络结构。

关 键 词：interphase nucleus F_actin TRITC_phalloidin cytochalasin D confocal laser scanning microscopy Allium sativum 

分 类 号：S633.401[农业科学—蔬菜学] Q942[农业科学—园艺学]