圈卷产色链霉菌尼可霉素生物合成基因-sanK的克隆及功能研究  

Cloning and Function of sanK-a Gene Involved in Nikkomycin Biosynthesis of Streptomyces ansochromogenes

作　　者：聂丽平[1] 李文利[1] 谭华荣[1] 

机构地区：[1]中国科学院微生物研究所

出　　处：《生物工程学报》2000年第6期667-670,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金重点项目!( 3 983 0 0 10 );国家杰出青年科学基金!( 3 992 5 0 0 2 )资助

摘　　要：利用染色体步移策略 ,以尼可霉素生物合成相关的基因片段为探针 ,从圈卷产色链霉菌中克隆到了一个大约 10kb的DNA片段。对其中 1 8kb的PvuII SacII片段进行了序列分析 ,结果表明 :此片段中含有一个具有1170个核苷酸的完整开放阅读框 ,起始密码子为 44 7位的ATG ,终止密码子为 16 14位的TGA ,推测其编码一个389个氨基酸的蛋白质产物。利用BLASTX程序进行的分析揭示 ,此基因编码一个肌氨酸单体氧化酶。基因功能研究结果表明 ,此基因与圈卷产色链霉菌尼可霉素生物合成直接相关 ,命名为sanK。The chromosome walking strategy has been used in this experiment and a 10kb DNA fragment was cloned from the cosmid library of \%Streptomyces ansochromogenes\% using a partial DNA fragment involved in the nikkomycins biosynthesis as a probe.The nucleotide sequence of 1.8kb Pvu II\| Sac II DNA fragment was determined and analysed.The result indicated that the 1170bp DNA fragment contains one complete open reading frame (ORF).The start codon is ATG at 447 bp position,and the stop codon is TGA at 1614bp position,the predicted product is a monomeric sarcosine oxadase using BLASTX programme.The experiment of gene disruption showed that the gene is closely related to nikkomycins biosynthesis of \%Streptomyces ansochromogenes.\%This gene was designated as \%sanK.\%

关 键 词：圈卷产色链霉菌 尼可霉素 生物合成 

分 类 号：Q939.92[生物学—微生物学]