利用RAPD分子标记评价仲彬草属的种间关系(英文)  

Relationships among Kengyilia species assessed by RAPD markers

作　　者：周永红[1] 郑有良[1] 杨俊良[1] 颜济[1] 贾继增[2] 

机构地区：[1]四川农业大学小麦研究所,四川都江堰611830 [2]中国农业科学院作物品种资源研究所,北京100081

出　　处：《植物分类学报》2000年第6期515-521,共7页Acta Phytotaxonomica Sinica

基　　金：四川省教育厅自然科学基金资助

摘　　要：利用随机扩增多态性DNA(RAPD)技术分析了 1 4种仲彬草属Kengyilia植物的种间关系。对 34个OPRON公司十聚体随机引物进行多态性筛选 ,2 0个 (58.8% )能产生多态性。 1 4个引物产生的 1 1 2个DNA片断 ,用于计算种间Jaccard遗传相似性系数分析 ,在NTSYS程序中利用UPGMA构建系统发育树状图。分析结果表明 :(1 ) 1 4个Kengyilia物种存在较大的遗传多样性 ;(2 )青藏高原的物种与新疆的物种的RAPD变异极大 ;(3)形态相似、地理分布一致的物种有一定的亲缘关系 ,聚类在一起 ;(4)RAPD结果与形态学和细胞学等分析结果一致。RAPD分析方法将为Kengyilia系统分类提供DNA水平上丰富的资料。WT5BZ]To assess the interspecific relationships in Kengyilia, fourteen species of Kengyilia were used for RAPD assay. Of the 34 random primers tested, twenty (58.8%) produced polymorphic products. A total of 112 products amplified from 14 primers were selected for RAPD analysis. Eighty three (74.1%) amplified products were found to be polymorphic among 14 species. Data were used to generate Jaccard's similarity coefficients and to construct a dendrogram using UPGMA in the NTSYS computer programs. It is concluded from this study that: (1) There was great genetic diversity among the 14 species of Kengyilia; (2) Distinct RAPD variations existed between the species from the Qinghai Xizang Plateau and those from Xinjiang; (3) The species with similar morphological characters and the species from the same areas or neighboring geographical regions were clustered together; (4) RAPD results are comparable with those obtained from studies on morphology and cytology. RAPD assay is a useful additional method for assessing the relationships among the species in Kengyilia.

关 键 词：仲彬草属 RAPD分析 种间关系 遗传变异 

分 类 号：Q949[生物学—植物学]