温度对野蚕黑卵蜂寄主利它素的影响  

Influence of temperatures on the activity of host recognition kairomone of Telenomus theophilae

作　　者：高其康[1] 胡萃[1] 

机构地区：[1]浙江大学,杭州310029

出　　处：《昆虫学报》2000年第4期373-379,共7页Acta Entomologica Sinica

基　　金：国家自然科学基金资助项目!(390 70 5 89);浙江省自然科学基金资助项目!(3982 5 5 )

摘　　要：利它素粗提液或涂利它素粗提液的人造卵经不同温度 (2 5℃、 6 0℃或 10 0℃ )处理 30min后进行生物活性测定 ,发现各温度下利它素仍对野蚕黑卵蜂有很强的引诱活性 ,其平均反应级数与常温 (2 5℃ )相比无明显差异 ,表现出一般蛋白质所没有的热稳定特性。低温 (4℃、 0℃、- 2 0℃或 - 70℃ )同样也能保存粗提液中利它素的生物活性 ,但发现能引起粗提液发生凝集 ,且0℃以下比 4℃能产生更多的凝集 ,使粗提液中利它素活性组分含量减少 ,- 2 0℃时 ,其活性组分的峰面积仅为对照的 13 1% ,表现出一般蛋白质所没有的低温敏感性 ,这一发现对利它素的快速、简捷、有效分离具有重要的指导意义。Kairomone activities of crude extract from female accessory glands of Bombyx mori after being treated for 30 min at 25℃, 60℃ and 100℃ were determined. The results showed that the extracted substance had strong attractive activity to Telenomus theophilae for all the 3 temperature treatments. The main grade of response at 60℃ or 100℃ showed no significant different from that at 25℃. This meant that the active substance was resistant to high temperature. At low temperature (4℃,0℃,-20℃ or -70℃ ) the substance kept active for eliciting T. theophilae, but these temperatures could cause deposition of the active component. The amount of deposit at 0℃ was larger than that at 4℃. At -20℃ the active component amounted to 13.1% of the control according to HPLC analysis. This finding of sensitivity to low temperature is very importance for simplifying methods for isolation and purification of the kairomone.

关 键 词：野蚕黑卵蜂 利它素 温度 引导活性 

分 类 号：S884.7[农业科学—特种经济动物饲养]