红麻质核互作型雄性不育相关基因atp1的克隆与分析  

Cloning and Sequence Analysis of Gene atp1in Cytoplasmic-Nuclear Male Sterile Line of Hibiscus cannabinus

作　　者：陈艳翠[1] 黄思齐[1] 李建军[1] 唐慧娟[1] 陈安国[1] 李德芳[1] 

机构地区：[1]中国农业科学院麻类研究所,湖南长沙410205

出　　处：《中国麻业科学》2013年第2期63-68,共6页Plant Fiber Sciences in China

摘　　要：从雄性不育相关基因克隆入手,开展红麻雄性不育机理的研究。根据不同物种atp1基因的保守序列设计引物,在红麻质核互作型雄性不育保持系中通过RACE克隆得到atp1基因cDNA全长,全长为2315 bp,和其他物种相比较,其同源性大于94%。该研究为下一步构建表达载体进行转基因表达研究提供了基础,为揭示红麻雄性不育的分子机理提供了一定依据。Gene cloning was conducted to study the mechanism of male sterility in kenaf （Hibiscus cannabinus）. According to the conserved sequence of gene atpl in different species, primers were de- signed. Full length of cDNA of gene atpl were cloned from the cytoplasmic - nuclear male sterile maintai- ner line by using the method of RACE. The gene was 2315 bp in length. This gene shares more than 94% identity of the sequence compared with other species. This study would provide the foundation for studying transgenic express of atpl by constructing expression vector, which will supply certain basis to reveal the mechanism of male sterility in kenaf.

关 键 词：红麻 质核互作雄性不育 基因克隆 

分 类 号：S563.5[农业科学—作物学]