原花青素与戊二醛共交联心脏瓣膜的相容性  

Compatibility characteristics of acellular porcine heart valve matrix co-crosslinked with procyanidins and glutaraldehyde

作　　者：马旭兵[1] 翟万银 张嘉敏[3] 朱自严[3] 张红锋[1] 常江 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]中国科学研究院上海市硅酸盐研究所高性能与超微结构国家重点实验室,上海200050 [3]上海市血液中心,上海200051

出　　处：《华东师范大学学报（自然科学版）》2013年第2期92-102,共11页Journal of East China Normal University(Natural Science)

基　　金：国家自然科学基金(31070870)

摘　　要：采用8mg/mL原花青素交联脱细胞猪主动脉心脏瓣膜4h后,再经1.25mg/mL戊二醛交联44h.生物相容性检测结果显示,共交联瓣膜组细胞粘附率较戊二醛单独交联提高59%((78.75±8.7)%～(19.75±3.27)%)、无明显溶血现象((0.75±0.36)%)、血小板粘附量较戊二醛单独交联显著减少((193±15.5)个vs(292.6±24.93)个)、免疫原性低((49.33±6.3)%vs(95.27±5.26)%).研究结果表明,共交联瓣膜材料有良好的细胞相容性、较好的血液相容性和较低的免疫原性,是一种优良的生物瓣材料,有望应用于生物瓣制备.The clinical used bioprosthetic heart valves （BHVs） were prepared from biological tissues by glutaraldehyde （GA） crosslinking. But the clinical application of BHVs is limited mainly due to calcification, cytotoxicity and remaining immunogenicity. Procyanidins （PC） can crosslink acellular heart valve matrix （AHVM） through hydrogen bond formation and prevent AHVM from calcification. The aim of this study is to modify GA crosslinking method using PC co-crosslinking to produce a much ideal material with higher compatibility for BHV preparation. Porcine aortic AHVM was crosslinked by 8 mg/mL PC for 4 h and then by h 25 mg/mL GA for 44 h. The co-crosslinked AHVM was tested for cytocompatibility, blood compatibility and im- munogenicity. The results showed that the in vitro adhesion rate of valvular interstitial cells on co-crosslinked AHVM was increased by 590/00 （78.75% -19.75 %）when compared with that of GA-crosslinking. There was no difference in hemolysis between co- and GA- or PC-crosslinked AHVM. The co-crosslinked AHVM exhibited significant anti-thrombosis effect （（193 ± 15.5） platelets） when compared with that of GA-crosslinked （（292.6 ± 24. 93） platelets）. Furthermore, co-crosslinked AHVM displayed lower immunogenicity than that of GA-crosslinked （（49.33 ±6.3）% vs （95. 27 ± 5. 26）%, cell adhesive rate）. These results suggest that cocrosslinked AHVM has high potential to be used for preparation of BHVs.

关 键 词：原花青素 戊二醛 交联 心脏瓣膜 相容性 

分 类 号：Q819[生物学—生物工程]