抗耐甲氧西林金黄色葡萄球菌菌株的筛选与鉴定  

Screening and Identification of Strains with Anti-methicillin-resistant Staphylococcus aureus Activity

作　　者：江娟[1] 邹远军[1] 张云玲[1] 刘红[1] 胡少南[1] 郑一敏[1] 

机构地区：[1]重庆理工大学药学与生物工程学院,四川重庆400054

出　　处：《现代食品科技》2013年第4期788-791,共4页Modern Food Science and Technology

基　　金：科技部重大新药创制专项(2010ZX09401-306-2-23);重庆市科委项目(2009GJF10023)

摘　　要：通过对246株芽孢杆菌进行筛选,以期获得对耐甲氧西林金黄色葡萄球菌(MRSA)具抑制作用的菌株,抑菌结果表明,对耐甲氧西林金黄色葡萄球菌有抑制作用的菌株有5株,其中菌株1012-4的抑菌活性最强;为确定菌株种属,对优选菌株进行形态特征观察、生理生化特征鉴定以及API50CHB鉴定,初步确定1012-4为枯草芽孢杆菌;为进一步确证菌株类型,实验采用了16S rDNA基因测序,序列结果表明,菌株1012-4为芽孢杆菌属枯草芽孢杆菌种;经反复确证,确定菌株1012-4为优势菌株,抗菌活性显著,为新型抗MRSA药物的研发提供了菌株来源。In this research, strains with antibacterial activity on Methicillin-resistant Staphylococcus aureus.246 tested strains was screened and identified, Antibacterial test results showed that five strains had antibacterial activity, among which strain 1012-4 showed the strongest activity. Morphological, physiological and biochemical characterization, as well as API50 CI-IB method, were adopted to determine the type of strain 1012-4. Preliminary results suggested that the stain 1012-4 was of Bacillus subtilis species. Through 16S rDNA gene sequence alignment, the strain 1012-4 was further identified as the genus Bacillus and Bacillus subtilis species. Verification test confirmed that, the strong antibacterial activity of strain 1012-4. This research provided new strain source for the research and development of new anti-MRSA drugs.

关 键 词：耐甲氧西林金黄色葡萄球菌 芽孢杆菌 筛选 鉴定 

分 类 号：R378[医药卫生—病原生物学]