一氧化氮对莴苣种子萌发过程中部分物质代谢的影响  

Effect of nitric oxide on substances metabolism of lettuce (Lactuca sativa L.) during seed germination

作　　者：杨北宁[1] 张凤芝[1] 苏国兴[1] 

机构地区：[1]苏州大学基础医学部基础医学与生物科学学院,江苏苏州215123

出　　处：《江苏农业学报》2013年第2期394-398,共5页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31171467)

摘　　要：以莴苣(Lactuca sativa L.)品种挂丝红种子为材料,用一氧化氮供体硝普钠(SNP)和一氧化氮生物合成抑制剂N-硝基-L-精氨酸甲酯(L-NAME)浸种,研究在莴苣种子萌发过程中内含物质和水解酶活性的变化。结果表明,浸种处理36 h,0.10 mmol/L SNP处理下莴苣种子萌发率显著高于0 mmol/L、0.05 mmol/L和0.20 mmol/LSNP处理(P<0.05);在浸种处理12～36 h,0.10 mmol/L SNP处理对种子萌发的促进作用最为显著,48 h后这种促进作用逐渐减弱;1.00 mmol/L L-NAME浸种可显著抑制种子的萌发率。0.10 mmol/L SNP处理可显著降低莴苣种子淀粉含量(P<0.05)和增加可溶性糖含量(P<0.05);1.00 mmol/L L-NAME处理可显著抑制淀粉的降解(P<0.05),且可溶性糖含量显著下降;0.10 mmol/L SNP处理下莴苣种子α-淀粉酶、硝酸还原酶和脂肪酶的活性随处理时间的延长而逐渐增加,β-淀粉酶活性在浸种36 h时达最大值后逐渐下降;1.00 mmol/L L-NAME处理下莴苣种子的β-淀粉酶、α-淀粉酶、硝酸还原酶和脂肪酶活性均受到抑制。Lettuce （Lactuca sativa L. ） Guasihong was used as a material to study some subtances metabolism and hydrolase activities of lettuce seed soaked in nitric oxide（NO） inhibitor L-NAME and different concentrations of NO donor sodium nitroprusside（SNP） during seed germination. The results indicated that the germination rate of lettuce seeds soaked in 0.10 mmol/L SNP for 36 h was higher than those in 0 mmol/L, 0.05 mmol/L, 0. 20 mmol/L SNP. The seed germination was improved with 0.10 mmol/L SNP for 12-36 h, but declined after 48 h. 1.00 mmol/L L-NAME significantly inhibited the seed germination. The starch content of lettuce seed significantly decreased and soluble sugar significantly increased in 0. 10 mmol/L SNP treatment. The starch degradation was inhibited and the soluble sugar content markedly declined in 1.00 mmol/L L-NAME treatment. The activities of a-amylase, nitrate reductase and lipase were increased with the extension of time, and IS-amylase activity reached the peak after being soaked for 36 h and then declined. The activities of β-amylase, α-amylase, nitrate reductase and lipase dropped in 1.00 mmol/L L-NAME treatment.

关 键 词：莴苣 一氧化氮 物质代谢 种子萌发 

分 类 号：S636.204.1[农业科学—蔬菜学]