“仙灵骨葆”灌胃给药大鼠后吸收入血成分的HPLC-MS/MS分析  

HPLC-MS/MS Analysis of the Absorbed Components in Serum after Intragastric Administration of Xianlinggubao to Rats

作　　者：秦子飞[1] 姚志红[1,2] 戴毅[1,2] 耿剑亮[3] 林舒颖[1] 吴肖蒙[1] 姚新生[1,2,3] 

机构地区：[1]暨南大学药学院,广东广州510632 [2]中药药效物质基础及创新药物研究广东省高校重点实验室,广东广州510632 [3]沈阳药科大学天然药物化学教研室,辽宁沈阳110016

出　　处：《分析测试学报》2013年第4期420-426,共7页Journal of Instrumental Analysis

基　　金：国家自然科学基金重大国际合作项目(81220108028);国家自然科学基金面上项目(81073003);广东省科技计划项目(2011B031700068)

摘　　要：采用高效液相色谱串联质谱法(HPLC-MS/MS),对中药复方制剂"仙灵骨葆"经大鼠灌胃给药后吸收入血的成分进行分析。以甲醇-乙腈(2∶1,体积比)对血清样本进行前处理,采用C18色谱柱,以乙腈-水(各含0.1%甲酸)为流动相进行梯度洗脱,选择正离子采集模式下的一级、二级质谱全扫描,对"仙灵骨葆"给药后血清、空白血清及"仙灵骨葆"制剂进行液相色谱串联质谱对比分析,根据色谱峰保留时间及质谱碎裂规律,结合文献和空白血清加入对照品的相关信息,确认了"仙灵骨葆"13个吸收入血原型成分并推测了2个代谢物,这些入血成份主要来源于淫羊藿、补骨脂、续断和知母四味组方中药,提示这四味中药可能对该复方体内发挥药效的贡献较大。上述研究可为揭示该复方在体内抗骨质疏松药效物质提供一定实验依据。A high performance liquid chromatography - tandem mass spectrometric （ HPLC - MS/MS ） method was developed for the analysis of the absorbed components in serum after intragastric administra- tion（ i. g. ） of Xianlinggubao （XLGB）, a kind of traditional Chinese medicine prescription, to rats Methanol -acetonitrile（2 ： 1 ） was used for serum sample pretreatment. The separation of components was achieved on a Cls column by gradient elution with acetonitrile （containing 0. 1% formic acid） - 0. 1% formic acid water solution as mobile phase. The ion acquisition was performed by full scan MS and MS/MS in positive ion mode. The serum after i.g. XLGB to rats, blank serum of rats and XLGB were analyzed by HPLC -MS/MS. 13 prototype compounds were confirmed and 2 metabolites were i- dentified in serum by comparing the retention time and mass spectrometry fragmentation characteristics with that of reference substance added in blank serum as well as literatures, which mainly derived from Herba Epirnedii Brevicornus, Fructus Psoraleae, Radix Dipsaci and Rh/zoma Anemarrhenae. It revealed that these four eompositive herb medicines contribute more to the effect of XLGB. The analysis of the absorbed components of Xianlinggubao in serum after administration would provide an experimental ha- sis for revealing the in vivo bioactive components of Xianlinggubao in anti-osteoporosis.

关 键 词：仙灵骨葆 吸收入血成分 HPLC—MS MS 抗骨质疏松药效物质 大鼠 

分 类 号：O657.63[理学—分析化学] R283.65[理学—化学]