两种紫花苜蓿苗期耐盐生理特性的初步研究及其耐盐性比较  

A study on physiological characteristics and comparison of salt tolerance of two Medicago sativa at the seedling stage

作　　者：刘晶[1] 才华[1] 刘莹[1] 朱延明[1] 纪巍[1] 柏锡[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《草业学报》2013年第2期250-256,共7页Acta Prataculturae Sinica

基　　金：黑龙江省高校科技创新团队建设计划(2011TD005);国家自然科学基金(31171578);转基因生物新品种培育重大专项(2008ZX08004-002)资助

摘　　要：为研究2种紫花苜蓿:肇东苜蓿和农菁1号苜蓿的耐盐程度及其在盐胁迫处理下的生理反应规律,在1/2Hogland营养液水培条件下,用0,50,100,200,300,400mmol/L NaCl溶液模拟盐胁迫处理2种紫花苜蓿幼苗,观察记录各处理的盐害情况,分别测定胁迫前和胁迫3,6,9,12,15,21d的丙二醛(MDA)、叶绿素(Chl)和脯氨酸(Pro)含量,分析了不同胁迫程度和胁迫时间下相关生理指标的变化情况,并采用隶属函数法对二者的耐盐性进行了综合评价。结果表明,低浓度盐胁迫对其生长无显著影响,2种苜蓿具有较强的耐盐性,能够抵抗一段时间较高浓度(200mmol/L)的盐胁迫。随着浓度的增加,MDA含量逐渐升高,但随胁迫时间的延长,在200,300mmol/L处理下,2种苜蓿的MDA含量表现出先增加,后下降,然后又上升的动态变化趋势。随着胁迫浓度的增加和时间的延长,二者受到的盐害逐渐增大,Chl含量逐渐降低,Pro含量大量累积,但二者的Pro累积程度与它们的耐盐表现并不完全一致。综合评价结果表明,在100,200mmol/L NaCl胁迫下,农菁1号苜蓿比肇东苜蓿更耐盐;而300,400mmol/L NaCl处理时,肇东苜蓿的耐盐性大于农菁1号苜蓿。The salt tolerance of Medicago sativa cv.Zhaodong and M.sativa cv.Nongjing No.1 and their physiological responses under salt stress were studied in seedlings cultured in 1/2 Hogland solution supplied with NaCl at concentrations of 0,50,100,200,300 or 400 mmol/L.Salt injury was measured after the treatment.The content of malondialdehyde（MDA）,chlorophyll,proline before and after 3,6,9,12,15 and 21 days of salt stress were measured and analyzed.The salt tolerance of the two species was comprehensively evaluated by fuzzy subordinate function.There was no significant effect on the growth of two M.sativa at low concentrations of NaCl,showing they had a certain salt tolerance ability.The content of MDA increased with an increase of NaCl concentration.While under the stress of 200 and 300 mmol/L,the content of MDA increased initially then decreased,and then increased again after a prolonged time.With the concentration and duration of NaCl stress increasing,salt injury increased,the content of chlorophyll reduced,and the proline content was considerably increased.However,the accumulation of proline was not entirely consistent with salt-tolerant performance.The results of a comprehensive evaluation showed that the salt tolerance of M.sativa cv.Nongjing No.1 was higher than that of M.sativa cv.Zhaodong under the stress of 100 and 200 mmol/L NaCl,while under the treatment of 300 and 400 mmol/L,the salt tolerance of M.sativa cv.Zhaodong was higher than that of M.sativa cv.Nongjing No.1.

关 键 词：肇东苜蓿 农菁1号苜蓿 耐盐性 综合评价 

分 类 号：S541.9[农业科学—作物学]