柯萨奇病毒A16型VP1区基因变异和进化分析  

Analysis on variation and evolution of the VP1 region of Coxsackievirus A16

作　　者：任倩[1] 王世富[1] 张忠[2] 于爱莲[2] 张乐海[1] 

机构地区：[1]山东大学齐鲁儿童医院 [2]泰山医学院

出　　处：《中华实验和临床感染病杂志（电子版）》2013年第1期20-23,共4页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：济南市科学技术发展计划第一批项目(No.201201063)

摘　　要：目的分析2010～2011年本院分离的2株柯萨奇病毒A16(Cox16)VP1区核苷酸(氨基酸)变异和进化特征,探讨CoxA16基因变异与临床特征的相关性。方法选取CoxA16所导致的手足口病患儿的咽拭子标本,采用RT-PCR法扩增其VP1区3’-端核苷酸序列(约400bp),所得序列与近几年中国内地和1951～2010年世界范围流行的CoxA16分离株进行核苷酸序列比对,并分析VP1区氨基酸的变异。结果本研究中2例患儿均有典型的手足口病表现:发热,双手、足红色皮疹和口腔黏膜散在的疱疹,其中分离出JN2011-B11的患儿有轻微肝功能损害。此2株CoxA16均属于B1b基因型,与2008年中国株(QH0549T)的同源性最高,核苷酸和氨基酸的同源性分别为96.0%和99.1%。近11年中国内地CoxA16VP1区C-端氨基酸序列变异很小,仅在225、235和266三个位点出现。资料显示世界范围内2001年后的流行株以B1a和B1b为主。结论近11年来,中国内地CoxA16毒株VP1区基因的变异程度较低,核苷酸变异率为4%～12.7%。本研究所分离的2株Cox-A16毒株核苷酸和氨基酸同源性均较高,达97.7%和96.8%。该病毒基因变异与临床特征的相关性尚有待于进一步验证。Objective To analyze the characteristics of variation and evolution in the VP1 region of Coxsackievirus A16 （CoxA16） and investigate the relationship between the mutations and clinical features thorough the two virus isolates obtained from children with hand, foot and mouth diseases （HFMD） in 2010 and 2011 in our hospital. Methods Two throat swabs from children with HFMD caused by CoxA16 were selected and the 3＇-terminal regions of VP1 （about 400 bp） were amplified by RT-PCR technique. Nucleotide sequences of this study with the strains from the mainland of China in recent years and the epedmic ones worldly during 1951-2010 were aligned, then the amino acid variation of the VP1 region was analyzed. Results The two patients had typical manifestations of HFMD： fever, the red rash in hands and foot and herpes in oral mucosa, while one of them （JN2011-Bll） had a mild liver function damage. The two isolates identified were most closely with Blb genotypes. Nucleotide and amino acid homology of the two sequences with QH0549T were 96.0% and 99. 1%, respectively. In recent 11 years, there was little variation in VP1 C-terminal region of CoxA16 in mainland, which only occurred in 225,235 and 266 locus. Conclusions The variation of CoxA16 VP1 region was lower in recent 11 years in China's Mainland. The nucleotide variation rate was 4%-12.7%. In this study, the nucleotide and amino acid homology of the two Ji＇ nan strains were relatively high, up to 97.7% and 96.8%, respectively. The relationship between CoxA16 gene mutation and the clinical features needed to be further verified.

关 键 词：柯萨奇病毒A16型 VP1区 变异 进化 临床特征 

分 类 号：R440[医药卫生—诊断学]