不同T细胞亚群T细胞受体删除环含量的评价  

Evaluation of the content of T cell receptor excision circles in different T cell subpopulations

作　　者：孟庆才[1,2] 朱鏐娈 郝禹[1,2] 周海卫 董晓君[3] 李彤 赵红心[1] 孔雅娴 蔺洁[5] 曾辉[1,2] 

机构地区：[1]首都医科大学附属北京地坛医院,北京100015 [2]新发突发传染病研究北京市重点实验室 [3]上海交通大学 [4]北京中医药大学 [5]首都医科大学附属北京安贞医院

出　　处：《中华实验和临床感染病杂志（电子版）》2013年第1期32-34,共3页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：病毒活化TLR3信号抑制淋巴造血的机制研究(No.81101251);北京市卫生系统高层次卫生技术人才培养计划(No.2009-2-13)

摘　　要：目的评价脓毒症等疾病对胸腺输出功能的影响,建立T细胞受体删除环(TREC)的检测方法。方法构建TREC质粒标准品,应用实时定量PCR法绘制标准曲线。选择不同年龄健康人的血液样本,提取其外周血单个核细胞(PBMCs),通过流式细胞术分选出不同亚群的T细胞,检测TREC含量(拷贝/Tcell)。结果 10例健康人血液PBMCs分选的不同T细胞亚群均检出TREC。CD4^+CD45RA^+及CD8^+CD45RA^+初始T细胞亚群中TREC平均含量均高于相应CD45RO^+记忆T细胞亚群的含量,差异具有统计学意义(P<0.05),且均随着年龄增长呈逐步下降趋势。结论建立实时定量PCR检测TREC的方法,用于评价胸腺T细胞的输出。Objective To evaluate the influence of the resent thymic emigrant by sepsis disease, a method to detect T cell receptor excision circle （TREC） was found. Methods The TREC plasmid standard sample was established and the standard curve was drawn by real-time PCR. Peripheral blood mononuclear cells （PBMCs） were separated from healthy peripheral blood and T cell subpopulations were sorted by flow cytometer. Real-time PCR was applied to detect the content of TREC （copies/cell） in these T cell subpopulations. Results The detectable rate of TREC was 100% （10/10）. The content of TREC in CD4＋ CD45RA＋ T and CD8＋ CIM5RA＋ T cells were significantly higher than that in CD4＋ CD45RO＋ T ceils and CD8 ＋ CD45RO＋ T cells （P 〈 0.05 ）, respectively. The content of TREC in these T cell subpopulations gradually declined with the age increasing. Conclusions The method that could be applied to evaluate the recent thymic emigrant was established by detecting the content of TREC in T cell subpopulations with real-time PCR.

关 键 词：T细胞受体删除环 胸腺输出 T细胞亚群 

分 类 号：R459.7[医药卫生—急诊医学]