低氧浓度与常规氧浓度对胚胎早期发育及妊娠结局的影响  

Effect of low and ambient oxygen concentration on early embryo development and pregnancy outcome

作　　者：牛子儒[1] 马慜悦[1] 岳晓静[1] 张士慧[1] 陆翀曌[1] 王辉[1] 舒军萍[1] 彭红梅[1] 姚元庆[1] 

机构地区：[1]解放军总医院妇产科生殖中心,北京100853

出　　处：《解放军医学院学报》2013年第4期340-343,共4页Academic Journal of Chinese PLA Medical School

基　　金：全军"十二五"重点项目(BWS11J058)~~

摘　　要：目的比较低氧浓度(5%O2)与常规氧浓度(20%O2)胚胎培养在体外受精(in vitro fertilization,IVF)或卵胞浆内单精子注射(Intracytoplasmic sperm injection,ICSI)中对胚胎早期发育及妊娠结局的影响。方法本中心195例IVF/ICSI周期接受常规促排卵,取卵后随机分为低氧浓度组与常规氧浓度组分别培养,观察其受精情况、胚胎发育情况、冷冻情况及妊娠结局。结果低氧组共纳入周期101例,常氧组94例。两组正常受精率、多精受精率、D1卵裂率、D2D3优胚率比较均无统计学差异(P>0.05);两组新鲜移植率、胚胎冷冻率、解冻移植率比较均无统计学差异(P>0.05);两组新鲜移植周期妊娠率,低氧组高于常氧组(χ2=3.963,P<0.05),两组解冻移植周期妊娠率相比无统计学差异。结论低氧浓度胚胎培养可一定程度提高IVF/ICSI妊娠结局,但仍需大样本的随机对照研究进一步证实。Objective To compare the effect of low and ambient oxygen concentration on early embryo development and pregnancy outcome after in vitro fertilization（IVF） and intracytoplasmic sperm injection（ICSI）.Methods One hundred and ninety-five pregnant women after IVF and ICSI who underwent routine ovulation promotion in our center were randomly divided into low oxygen concentration group（n=101） and ambient oxygen concentration group（n=94）.Their ova were cultured with oxygen a low concentration and at an ambient concentration,respectively,to observe their fertilization,embryo development and pregnant outcome.Results No significant difference was found in fertilization rate,multiple fertilization rate,D1 cleavage rate,D2 and D3 embryo scores,fresh implantation rate,frozen and thaw rate between the two groups（P 0.05）.The pregnant rate in fresh implantation cycle was higher in low oxygen group than in ambient oxygen group（χ2=3.963,P 0.05）.No significant difference was found in pregnant rate in thaw implantation cycle between the two groups（P 0.05）.Conclusion Embryo cultured at low oxygen concentration can improve the pregnancy outcome after IVF and ICSI,which should be further confirmed in random controlled study with a large sample.

关 键 词：胚胎培养 氧浓度 体外受精 卵胞浆内单精子注射 

分 类 号：R715[医药卫生—妇产科学]