干扰组蛋白去甲基化酶基因表达对Ishikawa细胞增殖凋亡的影响  

Interference with JARID1A gene expression on proliferation and apoptosis of Ishikawa cells

作　　者：郑金丹[1] 刘丽丽[2] 

机构地区：[1]辽宁医学院,辽宁锦州121000 [2]辽宁医学院第一附属医院,辽宁锦州121000

出　　处：《解放军医学院学报》2013年第4期389-391,404,共4页Academic Journal of Chinese PLA Medical School

基　　金：辽宁省自然科学基金(201102134);锦州市科学技术计划项目(12A1E35)~~

摘　　要：目的研究SiRNA干扰组蛋白去甲基化酶(JARID1A)基因表达对人子宫内膜癌Ishikawa细胞增殖凋亡的影响。方法 Ishi-kawa细胞转染JARID1A特异性的SiRNA;将Ishikawa细胞分成sicon组、sicon+e2组、siJARID1A组和siJARID1A+e2组,RT-PCR检测四组细胞孕激素受体(progesterone receptor,PR)mRNA表达,Western Blot检测四组细胞PR蛋白,MTT法观察细胞增殖,流式细胞术检测细胞周期。结果干扰JARID1A能显著上调PR表达,E2可进一步上调PR表达(P<0.01);E2能促进Ishikawa细胞增殖,转染SiRNA 48 h后细胞增殖受到抑制;转染SiRNA抑制细胞G1、G2/M期(P<0.05)。结论 JARID1ASiRNA能有效抑制Ishikawa细胞增殖。Objective To study the interference with siRNA JARID1A gene expression on proliferation and apoptosis of Ishikawa cells in endometrial cancer.Methods Ishikawa cells,transfected with JARID1A siRNA,were divided into sicon group,sicon e2 group,siJARIDIA group,and siJARID1A e2 group.Expressions of progestogen receptor（PR） mRNA and protein in the 4 groups were detected by RT-PCR and Western blot,respectively.Proliferation and apoptosis of Ishikawa cells were assayed by MTT and flow cytometry,respectively.Results Interference with JARID1A could significantly up-regulate the PR expression.E2 could further up-regulate the PR expression and promote the proliferation of Ishikawa cells（P 0.01）.The proliferation of Ishikawa cells was inhibited at G1 and G2/M phase 48 h after transfected with siRNA（P 0.05）.Conclusion JARID1A siRNA can effectively inhibit the proliferation and apoptosis of Ishikawa cells.

关 键 词：子宫内膜癌 小干扰RNA 组蛋白去甲基化酶 

分 类 号：R711.7[医药卫生—妇产科学]