食物种类和密度对萼花臂尾轮虫休眠卵形成的影响  

EFFECT OF BOTH ALGAE COMPONENTS AND DENSITIES ON THE FORMATION OF RESTING EGGS OF THE FRESHWATER ROTIFER,BRACHIONUS CALYCIFLORU

作　　者：席贻龙[1] 黄祥飞[1] 

机构地区：[1]中国科学院水生生物研究所,武汉430072

出　　处：《动物学报》2000年第1期27-36,共10页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金项目 !(No 3 9870 15 8);中国科学院九五重大项目! (No KZ95 1 A1 10 2 0 1)资助课题

摘　　要：采用种群累积培养法 ,研究了藻类食物的种类和密度对萼花臂尾轮虫休眠卵形成的影响。结果表明 ,萼花臂尾轮虫的休眠卵主要在开始培养后的 6天内形成。在 0 1mg/ml的食物密度下 ,与蛋白核小球藻相比 ,斜生栅藻或斜生栅藻和蛋白核小球藻以 1∶1(湿重 )组成的混合藻均能显著地提高休眠卵的产量和形成效率 ,同时也使轮虫种群中的平均混交雌体百分率和产休眠卵的混交雌体的平均产卵量明显增大 ,但对平均混交雌体受精率无显著影响。以蛋白核小球藻为食物时 ,随着其密度由 0 375× 10 7细胞 /毫升 (cells/ml)逐渐升高至 6 0× 10 7cells/ml,轮虫休眠卵产量极显著地增大 ,而休眠卵形成效率则极显著地降低 ;平均混交雌体百分率以 6 0× 10 7cells/ml组显著低于其它各密度组 ,平均混交雌体受精率则无显著差异。The effects of both algae components and densities on the formation of resting eggs of freshwater rotifer,Brachionus calyciflorus, obtained by hatching the resting eggs in sediment from Lake Donghu and culturing clonally in March, 1998, were studied using population accumulative culture method, under the conditions of temperature 25±1℃, intensity of illumination 300 lx and illumination period L∶D=16∶8. Two species of algae, Chlorella pyrenoidosa and Scenedesmus obliquus were cultured by the method of Li Shanghao's . The results showed that the resting eggs were produced mainly during the initial six days of the culture. At the food density of 01 mg/ml, the production and efficiency of formation of resting eggs of the rotifers fed Sobliquus or mixed algae of 1∶1 mixture (wet weight) of S.obliquus and C.pyrenoidosa were 71245±6514 ind/(10ml6d) and 11874±1084 ind/mg diet, or 58400±14820 ind/(10ml6d) and 9733±2470 ind/ mg diet, respectively.Rotifers fed C.pyrenoidosa produced significantly more eggs: 1643333±401040 ind/(10ml6d) and 2739±668 ind/mg diet, respectively.The remarkable increases of mixis rate in the populations and mean number of resting eggs produced by a resting eggproducing mictic female were also observed.But there was no significant difference of fertilization rate among the rotifers fed three types of diet. Using C.pyrenoidosa as the rotifers' food, with the increase of food density the production of resting eggs increased very significantly, but the efficiency of resting egg formation decreased very significantly.The relationship between the production of resting egg [y, ind/(10ml6d)] and the algae food density (x, ×107cell/ml) fitted to the equation: y=78524-5929x.

关 键 词：萼花臂尾轮虫 

分 类 号：Q958[生物学—动物学]