逆行电刺激对相邻背根节PPT mRNA表达的影响  

INFLUENCE OF ANTIDROMIC ELECTRIC STIMULATION ON PREPROTACHYKININ mRNA EXPRESSION IN ADJACENT DORSAL ROOT GANGLIA IN RATS

作　　者：李秀华[1] 赵晏[1] 史文春 李信民 王会生 

机构地区：[1]西安医科大学神经生物学研究中心 [2]第一临床医学院,西安710061

出　　处：《神经解剖学杂志》2000年第1期71-74,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金! ( No. 3 9470 841);国家攀登计划资助! ( No.JL93 0 2 1)

摘　　要：给予大鼠左侧胸 9脊神经背侧皮支或其外周断端较强电刺激后 ,用原位杂交方法观察相邻节段背根节 PPT m RNA表达的变化。结果表明 ,电刺激完整神经后 2 4h,同侧胸 8、胸 9和胸 10背根节 PPT m RNA表达的阳性细胞数明显高于对照组 ( P<0 .0 5 )。电刺激断离中枢的神经外周端后 2 4h,同侧胸 8和胸 10背根节 PPT m RNA表达的阳性细胞数明显高于对照组 ( P<0 .0 5 )。表明较强的逆行电刺激 ,可以在外周通过跨节段传递信息 ,引起相邻节段背根节的 PPT m RNA表达的增强。提示由 PPT生成的By in situ hybridization histochemistry, the changes of preprotachykinin(PPT) mRNA expression were examined in the neurons of adjacent thoracal dorsal root ganglia(DRG) after a strong electric stimulation to an intact dorsal cutaneous branch and the distal part of the cut left ninth thoracal spinal nerve of rat. There was a significant increase of the number of neurons expressing PPT mRNA in the ipsilateral 8th, 9th and 10th thoracal DRGs 24 h after the electric stimulation to the intact dorsal cutaneous branch. The same increase was found in the ipsilateral 8th and 10th thoracal DRGs given electric stimulation to the distal part of cut spinal nerve branch. No change was found in the DRGs on the contralateral side of these animals. The present results showed that the strong antidromic electric stimulation could strengthen the biosynthesis of PPT mRNA in adjacent DRGs. These findings suggested that there was information transmission across segments between sensory nerve endings and some bioactive substances such as SP might play important roles in this information transmission.

关 键 词：电刺激 原位杂交 跨节段信息传递 背根节 大鼠 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]