N-乙酰半胱氨酸对肺纤维化小鼠肺泡灌洗细胞核因子κB动态表达的影响  

Effects of N-acetyicysteine on dynamic expression of nuclearfactor-kappaB in bronchoalveolar lavage cells of mice with pulmonary fibrosis

作　　者：刘卫青[1] 秦秉玉[1] 朱敏[2] 张晓晔[3] 

机构地区：[1]郑州大学人民医院重症医学科,450003 [2]郑州大学人民医院重症呼吸科 [3]中国医科大学附属盛京医院肿瘤内科

出　　处：《中华实验外科杂志》2013年第4期743-745,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30100076、30570812）;辽宁省自然科学基金资助项目（20042085）

摘　　要：【摘要】目的观察在博莱霉素（BLM）致小鼠肺间质纤维化（IPF）病理过程中，N－乙酰半胱氨酸（NAC）对肺泡灌洗细胞核因子KB（NF—KB）动态变化的影响。方法75只C57BL／6小鼠随机分3组，BLM组25只气管注射BLM5mg／kg，NAC组25只于注射BLM前5d开始每天给予NAC250mg／kg直至取材，对照组25只气管注射生理盐水，于1、3、7、14、28d处死，右肺行支气管肺泡灌洗3次，灌洗细胞测p65及其mRNA的表达；左肺行苏木素．伊红（HE）染色和Masson染色。结果在实验7、14、28d，BLM组肺组织病理可见大片实变及胶原沉积，而NAC组明显减轻；在气管内灌人BLM后1d，BLM组p65蛋白与mRNA表达水平（％）分别为43．45±3．60、42．24±2．59，NAC组p65蛋白与mRNA表达水平（％）分别为21．43±2．86、25．214-3．03，肺泡灌洗细胞核p65蛋白表达与细胞质p65mRNA表达均明显增强，随着病程的进展表达强度减弱，3组小鼠NF-KBp65表达水平差异有统计学意义，且NAC组NF—KBp65的表达低于BLM组（P〈0．05）。、结论在BLM致肺间质纤维化的早期病理过程中，支气管肺泡灌洗细胞NF—KB的表达明显增强，NAC可能通过抑制NF—KBp65mRNA转录，减少NF—κβp65蛋白的表达，进而减轻BLM所致的肺纤维化程度。Objective To investigate the effects of N-acetylcysteine （NAC） on dynamic expression of nuclear factor-KB （NF-KB） in mice with bleomycin （BLM）-induced interstitial pulmonary fibrosis. Meth- otis A total of 75 C57BL/6 naice were randomly allocated into three groups. The mice in BLM group （n = 25） and control group （n = 25） received resPectively intratracheal instillation of BLM and saline, and those in NAC group were given NAC once a day via the esophagus 5 days before BLM treatment in 25 mice until sampling. On the day 1, 3, 7, 14, and 28, the left lung tissue was collected for hematoxylin and eosin （HE） and Masson staining, and bronchoalveolar lavage performed 3 times in the right lungs and bronchoalve- olar lavage cells to detect the NF-KB p65 expression. Results On the day 7, 14 and 28, as compared with BLM group, excessive lung solidation and collagen sediment in NAC group was significantly reduced. On the day 1 after the intratracheal instillation of BLM, the positive expression rate of p65 protein and mRNA in BLM group was 43.45± 3. 60 and 42. 24 ± 2. 59, and that in NAC group was 21.43± 2. 86 and 25.21 ± 3. 03, respectively. The p65 protein expression in the nuclei and p65 mRNA expression in the plasma in the bronehoalveolar lavage cells were significantly higher, and decreased with the prolongation of the disease course. There was significant difference in level of NF-κβ p65 expression in the bronchoalveolar lavage cells among 3 groups, and level of NF-κβ p65 expression in NAC group was lower than that in BLM group （P 〈 0. 05 ）. Conclusion In the process of BLM-indueed interstitial pulmonary fibrosis, the expression of NF-KB in bronehoalveolar lavage cells increases obviously. NAC may alleviate the extent of BLM-induced pulmonary fibrosis through inhibiting the transcription of NF-KB p65 mRNA and reducing the expression of NF-KB p65 protein.

关 键 词：N-乙酰半胱氨酸 博莱霉素 核因子-κB 肺间质纤维化 

分 类 号：R285.5[医药卫生—中药学]