串珠素对成纤维细胞增殖的影响及其与碱性成纤维细胞生长因子的关系  

Effect of pelercan on proliferation of fibroblasts and its relationship with basic fibroblast growth factor

作　　者：刘伟良[1] 褚言琛[1] 刘伟莉[1] 王进[1] 李东梅[1] 邹云雯[1] 

机构地区：[1]青岛大学医学院附属医院脊柱外科,266003

出　　处：《中华实验外科杂志》2013年第4期804-807,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81071482）

摘　　要：目的观察串珠素对成纤维细胞增殖的影响及其与碱性成纤维细胞生长因子（bFGF）的关系。方法建立模型，取大鼠瘢痕组织，行原代及传代培养。慢病毒干扰串珠素表达后，通过荧光定量聚合酶链反应（FQ—PCR）、Westernblot和噻唑蓝（M1Tr）法分别检测串珠素表达和细胞增殖率的变化。结果慢病毒感染后成纤维细胞串珠素mRNA表达降低（75．1±1．1）％，串珠素和bFGF的蛋白表达明显下调。低血清培养条件下，串珠素干扰组细胞增殖率比对照组下降（40．6±3．1）％。加入1μg／LbFGF后，串珠素干扰组增殖率差异无统计学意义（P〉0．05）。结论大鼠成纤维细胞串珠素表达下调后，bFGF表达亦减少，细胞增殖率下降并对bFGF的反应性降低。Objective To observe the effect of pelercan on proliferation of fibroblasts and its rela- tionship with basic fibroblast growth factor （bFGF）. Methods The rat model was established, and the scar connective tissue was collected and subjected to the primary cuhure of rat fibroblasts. Lentivirus was used to downregulate the expression of pelercan. Fluorescent quantitative polymerase chain reaction （ FQ-PCR ） , Western blotting and methyl thiazoi tetrazolium （MT＇F） assay were performed to detect the expression of per- leean and cell proliferation rate. Results After the lentivirus transfection, the perleean mRNA expression level in fibroblasts was reduced by （75. 1 ±1.1 ）% in perlecan-interfering group. In low-serum condition, the cell proliferation rate was decreased by （40. 6 ± 3.1 ） % in perlecan-＇interfering group. Treatment with 1 μg/L bFGF had no significant effect on the cell proliferation （ P 〉 O. 05 ）. Conclusion Infection of rat fi- broblasts with lentivirus down-regulated the expression of perlecan and bFGF. Cell proliferation rate and sensitivity of rat fibroblasts to bFGF were both reduced when perlecan was downregulated.

关 键 词：成纤维细胞 串珠素 碱性成纤维细胞生长因子 

分 类 号：R6[医药卫生—外科学]