青娥丸中三种药效成分的LC-MS/MS法测定  

Determination of Three Effective Components in Qing'e Pills by LC-MS/MS

作　　者：马改霞[1] 贾艳[1] 郭鹏飞[1] 黄素臻 苏小静[1] 

机构地区：[1]河北省廊坊卫生职业学院药理教研室,河北廊坊065001

出　　处：《中国医药工业杂志》2013年第4期382-385,共4页Chinese Journal of Pharmaceuticals

摘　　要：建立了液相色谱-串联质谱法同时测定青娥丸中的3种药效成分——松脂醇二葡萄糖苷、补骨脂素和异补骨脂素。采用Kromasil C18色谱柱,以甲醇-0.1%甲酸为流动相进行梯度洗脱,使用多反应监测(MRM)模式,监测离子对为m/z 700.5→m/z 235.4(松脂醇二葡萄糖苷)和m/z 187.1→m/z 131.1(补骨脂素和异补骨脂素)。3种成分的平均回收率为99.1%、99.0%和98.6%,RSD为1.33%、1.43%和1.27%。A LC-MS/MS method was established for the determination of three components, pinoresinol diglucoside, psoralen and isopsoralen in Qing＇e pills. A Kromasil C18 column was used with the mobile phase of methanol-0.1% formic acid for gradient elution. ESI was applied with multiple reaction monitoring （MRM） scanning. The transitions of m/z 700.5-m/z 235.4 （pinoresinol diglucoside） and m/z 187.1-m/z 131.1 （psoralen and isopsoralen） were monitored. Their average recoveries were 99.1%, 99.0% and 98.6%, with RSDs of 1.33%, 1.43% and 1.27%.

关 键 词：青娥丸 松脂醇二葡萄糖苷 补骨脂素 异补骨脂素 液相色谱-串联质谱 测定 

分 类 号：TQ460.72[医药卫生—药物分析学] O657.72[化学工程—制药化工]