鱼类消化道具有吸收外源生长激素的能力  

ABSORPTION OF RECOMBINANT COMMON CARP GROWTH HORMONE (rcGH) BY THE GASTROINTESTINAL TRACT OF FISH

作　　者：肖东[1] 陈松林[2] 严安生[3] 陈细华 邓文涛 

机构地区：[1]中山大学生命科学学院,广州510275 [2]长江水产研究所生物室,沙市434000 [3]华中农业大学水产学院,武汉430070

出　　处：《Zoological Research》2000年第2期103-107,共5页动物学研究（英文）

基　　金：农业部九五重点项目![农业部(渔)96-03]

摘　　要：采用草鱼生长激素夹心式酶联免疫吸附测定法 (ELISA) ,研究了重组鲤生长激素 (rcGH)经胃或肠灌注后 ,胡子鲶、大口鲶和银鲫血清GH水平的变化规律。胡子鲶、大口鲶直肠分别灌注rcGH ( 1 μg/g和 5μg/g)后 ,血清GH水平迅速升高 ,而在对照组胡子鲶和大口鲶血清中未检测到内源性的GH ,预示处理组血清GH水平的迅速升高是由rcGH从小肠内转移进血液引起的。胡子鲶胃内灌注rcGH ( 2 5 μg/g)后 ,在 48h内血清中未检测到外源性的rcGH和内源性的GH。银鲫前肠灌注rcGH ( 5 μg/g)后 ,33h内血清GH水平均极显著 (P <0 0 0 1 )或显著 (P <0 0 5 )高于对照组。本研究显示 :胡子鲶、大口鲶和银鲫消化道具有吸收外源GH的能力。Using the sandwich enzyme linked immunosorbent assay (ELISA) for grass carp ( Ctenopharyngodon idellas ) growth hormone and different administrations of rcGH,we investigated the possibility of rcGH absorption by gastrointestinal tract of walking catfish ( Clarias batrachus ),northern sheatfish ( Silurus soldatovi ) and crucian carp ( Carassius auratus gibelio ).The specific ELISA can recognize endogenous GH in grass carp,jian carp and crucian carp blood and exogenous rcGH in northern sheatfish and walking catfish blood,but not endogenous GH in northern sheatfish and walking catfish blood.Rectal administration of rcGH to walking catfish (1?μg/g b w ) and northern sheatfish (5?μg/g b w ) resulted in rapid and marked increase in serum GH levels,suggesting that rcGH be absorbed by the intestine into the blood.In contrast,exogenous rcGH in serum was undeteted over a period of 48?h after an intragastic administration of rcGH (25?μg/g b w ) to walking catfish.After an anterior intestine administration of rcGH (5?μg/g) to crucian carp,serum GH levels were significantly higher than the controls ( P <0 001 or P <0 05) over a period of 33?h post administration.

关 键 词：胡子鲶 大口鲶 银鲫 重组鲤生长激素 消化道 

分 类 号：Q959.4[生物学—动物学]