番茄下胚轴离体培养植株再生及其组织学观察  

Plant regeneration from hypocotyl explants cultured in vitro and histological observations in Lycopersicon esculentum Mill

作　　者：陈火英[1,2] 张建华[3] 钟建江[1] 俞俊棠[1] 

机构地区：[1]华东理工大学生物化工系 [2]上海交通大学植物科学系,上海201101 [3]上海交通大学植物科学系

出　　处：《西北植物学报》2000年第5期759-765,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：上海市教委"曙光计划"项目!( 98SG4 7)

摘　　要：研究了上海地区的主要栽培品种之一“鲜丰”番茄下胚轴离体培养过程中的激素调控 ,结果表明 :“鲜丰”番茄下胚轴进行离体培养过程中 ,MS培养基上附加不同浓度的生长素( IAA)和细胞分裂素 ( BA) ,对愈伤组织的形成影响不大 ,但对不定芽的分化有较大的影响 ,得出最佳培养基为 MS+ BA1 .0～ 2 .0 mg· L- 1+ IAA0 .2 mg· L- 1。用不同浓度的 ZT、BA、KT进行单因子芽器官的诱导实验 ,发现 ZT的作用力强于 BA和 KT,KT最弱 ;用不同浓度NAA、IAA、IBA、2 ,4- D进行发根培养实验 ,发现番茄的内源生长素浓度较高 ,用外植体直接发根外加生长素有一定的作用 ,若用不定芽扦插发根 ,不附加生长素也极易发根 ,故番茄的生根培养基为 1 /2 MS或 MS附加 IAA 0 .0 5～ 0 .1 mg· L- 1。另外 ,对有关细胞启动、分裂、分化以及器官发生的组织学观察表明 :番茄离体培养中不定芽通常发生在愈伤组织的周边区 ,也可起源于维管组织结节周围的形成层状细胞。不定根则由茎中柱鞘处发生。Tomato hypocotyls cut from cultivar ‘Xianfeng’ were cultured on MS medium supplemented with BA and IAA at different levels for callus induction and bud formation.The results showed that the effects of BA and IAA levels in media were less significant on callus formation,but more significant on adventitious shoot differentiation.The shoot induction rate was the highest on MS medium supplemented with BA 2.0 mg/L+IAA 0.2 mg/L.Cytohistological observations on callus and bud formation were carried out.Buds differentiated from calli were adventitious buds.Adventitious buds were generally formed in the peripheral parts of the calli,and originated from the cambium like cells surrounded by the vascular nodules.Adventitious roots originated from the pericycle.In addition,according to the study on effects of ZT,BA and KT on bud organogenesis from hypocotyl explants,ZT was the strongest and KT was the weakest.

关 键 词：番茄 离体培养 激素 形态发生 组织学观察 

分 类 号：S641.203[农业科学—蔬菜学]