钩端螺旋体外膜蛋白基因重组载体构建及在卡介菌中的表达  

Construction of Recombinant Vector for Expressing Leptospira Envelope Protein in BCG

作　　者：邱洪宇[1] 鲍朗[1] 晏菊芳[1] 谢勇恩[1] 徐恒[2] 冯云[1] 陈玮[1] 张会东[1] 张万江[1] 

机构地区：[1]华西医科大学钩体病研究室,成都610041 [2]四川大学生物科学学院,成都610064

出　　处：《中国生物制品学杂志》2000年第3期136-139,共4页Chinese Journal of Biologicals

基　　金：国家自然科学基金资助!批准号39670652

摘　　要：目的　研制一种高效广谱的新型钩体疫苗。方法　用PCR方法扩增致病性钩端螺旋体赖型 0 17株外膜蛋白OmpL1基因 ,将其克隆入大肠杆菌—分枝杆菌穿梭质粒载体pMV2 6 1和pMV36 1中。结果　筛选到两个重组载体pBQ1和pBQ2 ,通过电穿孔导入卡介菌 ,重组体经诱导表达了相对分子质量约为 35 0 0 0的融合蛋白。Objective To prepare a new broad spectrum leptospira vaccine. Methods OmpL1 gene was amplified from the genome of leptospira serovar Lai Strain 017 by PCR and cloned into E.coli BCG shuttle plasmids pMV261 and pMV361. Results Two recombinant vectors pBQ1 and pBQ2 were constructed and transformed to BCG by electroporation. The fusion protein with a molecular weight of 35 000 was expressed under the induction of high temperature (45℃).Conclusion Leptospira antigen protein can be effectively expressed in BCG.

关 键 词：钩端螺旋体 外膜蛋白 重组载体 卡介菌 

分 类 号：R377.5[医药卫生—病原生物学]