烟草生产霍乱毒素B亚单位疫苗的免疫分析  

Analysis of immunogencity of tobacco-derived cholera toxin B subunit vaccine

作　　者：王新国[1] 张艳红[1] 刘传暄[1] 高丽华[1] 肖成祖[1] 张国华[2] 方荣祥[2] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071 [2]中国科学院微生物研究所,北京100080

出　　处：《生物技术通讯》2000年第3期192-195,共4页Letters in Biotechnology

摘　　要：用纯化的CTB后腿肌肉注射免疫小鼠 ,每次 12 .5 μg ,每隔 14天加强一次 ,共注射三次 ,末次免疫后两周收集血清 ,免疫小鼠诱导产生了特异性抗CTB抗体。在CHO细胞中 ,免疫小鼠血清能够中和CT的细胞病变效应 ;小鼠肠结扎实验表明 ,抗CTB血清能够抑制CT结合细胞表面受体GM1神经节苷脂。Plant-derived CTB was used to immunize mice, and the serum samples were examined for CTB-specific antibodies. It indicated that the immunized mice produced significantly high titer of anti-CTB serum. The cytopathic effect of CT on CHO cell was neutralized by the serum, and the protective efficacy of immunization was assayed by mouse ileal loop ligation. All these results demonstrated that the antiserum showed protection against a bacterial enterotoxin.

关 键 词：霍乱毒素B亚单位 小鼠 疫苗 免疫 

分 类 号：R516.503[医药卫生—内科学]