转基因烟草生产霍乱毒素B亚单位的纯化  

Purification of cholera toxin B subunit from transgenic tobacco

作　　者：王新国[1] 张艳红[1] 刘传暄[1] 肖成祖[1] 张国华[2] 方荣祥[2] 

机构地区：[1]军事医学科学院生物工程研究所,北京100071 [2]中国科学院微生物所植物生物技术实验室,北京100080

出　　处：《生物技术通讯》2000年第2期103-106,共4页Letters in Biotechnology

摘　　要：提取转基因烟草叶片总蛋白 ,用经溴化氰活化的Sepharose 4B偶联有抗CTIgG色谱柱 ,得到了表达产物CTB蛋白质。经PAGE、Westernblot。CTB was purified from transgenic tobacco by affinity chromatography, and analyzed by SDS-PAGE, Western blot, immunodiffusion and immunoelectrophoresis. The results indicated that plant-derived CTB protein was antigenically indistinguishable from native CTB protein, and that oligomeric CTB molecules were the dominant molecular species isolated from transgenic tobacco leaves.

关 键 词：霍乱毒素B亚单位 纯化 转基因烟草 

分 类 号：Q78[生物学—分子生物学] Q510.3