磷酸钙法转染哺乳动物细胞SP2/0及其表达产物检测  

Transfection of mammalian cells SP2/0 using calcium phosphate method and detection of their expressions

作　　者：秦山[1] 黄春妍[1] 何芳[1] 刘丽[1] 赵连三[1] 

机构地区：[1]华西医科大学附属第一医院肝炎研究室,成都610041

出　　处：《生物技术通讯》2000年第2期115-117,共3页Letters in Biotechnology

基　　金：国家自然科学基金 (项目号 39670 670 );四川省科委"九五"攻关重大项目;四川省计委科研基金资助

摘　　要：将真核细胞表达质粒以磷酸钙介导法导入小鼠骨髓瘤细胞SP2 0 ,转染细胞经超声破碎后 ,分别以显色法及化学发光法检测其目的蛋白 (HBsAg) ,证实化学发光法的检测灵敏度高于目前常用的显色法 ,且其结果易于保存。对化学发光法的实验条件及磷酸钙转染法的有关影响因素进行了初步探讨。Bal b/c inbred mice osteomyoma cell line SP2/0 was transfected with eukaryotic?expression plasmids reconstructed in our laboratory. The transfected cells were broken up by ultrasonic and the supernatants were tested for HBsAg (expressed by the reconstructed plasmids) by chemiluminescence and chromogenic methods respectively. The result showed that the chemiluminescence method was better than that of chromogenic method and the former was easy to preservation. Furthermore, the conditions of chemiluminescence method and transfection method of calcium phosphate were optimized.

关 键 词：转染 哺乳动物细胞 表达产物 磷酸钙法 

分 类 号：Q786[生物学—分子生物学]