水稻白叶枯病广谱抗性基因Xa21导入两用不育系培矮64S  

Introduction of Wide Spectrum Rice Bacterial Blight Resistance Gene Xa21 into Two line Genic Male Sterile Rice Variety Pei'ai 64S

作　　者：赵彬[1,2] 王文明[1] 郑先武[1] 王春莲[3] 马伯军[1] 薛庆中[4] 朱立煌[1] 翟文学1 

机构地区：[1]中国科学院遗传研究所 [2]浙江大学农学系杭州310029 [3]中国农业科学院作物育种栽培研究所 [4]浙江大学农学系

出　　处：《生物工程学报》2000年第2期137-141,共5页Chinese Journal of Biotechnology

基　　金：国家高技术发展与计划资助项目!( 10 1 0 1 0 2 0 1)

摘　　要：以克隆的Xa21基因为外源基因,成熟胚愈伤组织为转化受体,应用农杆菌介导法对水稻两用型核不育系培矮64S进行转化,获46株转基因植株。PCR和Southern分析结果表明,Xa21已整合到受体基因组。用稻白叶枯病病原菌(Xanthomonasoryzaepv.oryzae)菲律宾小种6号接种鉴定,结果表明大多数转基因植株获得了抗病性。已整合的Xa21基因能够稳定地遗传,在所检测转基因株系的T1代中,Xa21基因显示3:1的分离。Agrobacterium mediated transformation of two line genic male sterile Indica rice variety Pei'ai 64S was conducted using a cloned gene,Xa21,as the foreign gene and mature embryo calli as the recipients.A total of 46 transgenic plants had been obtained.The PCR analysis and Southern blotting showed the integration of Xa21 gene into the genome the transgenic plants.Results of inoculation with philippine race 6 of Xanthomonas oryzae pv.oryzae indicated that most of transgenic plants obtained high resistance to rice bacterial blight disease (Xoo).Analyses of T 1 plants of the tested transgenic lines showed that integrated Xa21 gene could be steadily inherited and segregated in a 3:1 ratio.

关 键 词：水稻 白叶枯病 XA21基因 培矮64S 不育系 

分 类 号：Q78[生物学—分子生物学]