无血清培养昆虫细胞(BTI-Tn-5B1-4)的适应过程  

Adaptation of Insect Cells(BTI-Tn-5B1-4) to Serum free Culture

作　　者：戴琥[1] 赵佼[1] 谭文松[1] 杨曜中 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《生物工程学报》2000年第2期232-234,共3页Chinese Journal of Biotechnology

摘　　要：昆虫细胞培养是近年来迅速发展起来的动物细胞培养工程中的一个新领域。人们可以利用杆状病毒在昆虫细胞内的感染、复制,来大量生产昆虫病毒作为生物杀虫剂[1]。而昆虫细胞杆状病毒表达载体系统的建立,则可通过昆虫细胞的体外培养大量表达病毒携带的外源基因。实践证明,这...The insect cells(BTI Tn 5B1 4)were adapted,from the medium supplemented with 5% FBS,via 3%,2%,1% FBS,to grow in serum free medium by sequential adaptation for about three months.After adaptation,the cells could successfully grow in the specifically designed serum free medium with the viability above 90%.In addition,the effect of FBS concentrations and inoculum sizes on the insect cell growth was also investigated.

关 键 词：昆虫细胞培养 无血清培养 适应过程 

分 类 号：Q969.9[生物学—昆虫学]