慢生型大豆根瘤菌的遗传多样性研究  

STUDY ON GENETIC DIVERSITY OF SLOW GROWING RHIZOBIA OF GLYCINE MAX

作　　者：冯瑞华[1] 

机构地区：[1]中国农业科学院土壤肥料研究所,北京100081

出　　处：《应用与环境生物学报》2000年第2期176-181,共6页Chinese Journal of Applied and Environmental Biology

摘　　要：采用选择性扩增片断长度多态性 (简称AFLP)DNA指纹技术对来自泰国北部的 2 45株慢生型大豆根瘤菌进行遗传多样性的研究 .通过AFLP图谱揭示出该地区的慢生型大豆根瘤菌有较显著的遗传多样性 .从 2 45株中选择出 92个代表株用计算机进行的树状图分析结果表明 ,所分析的菌株在 82 %的相似性水平上聚类成 8个群 .对这 92个代表株的部分菌株和慢生型大豆根瘤菌的参比菌株进行多聚酶链反应 (PCR)扩增的 16SrDNA的 4种限制性内切酶长度多态 (简称 16SrDNAPCR RFLP)分析 ,得出 2个不同的 16SrDNAPCR RFLP类型的菌株 ,分别与慢生型大豆根瘤菌的参比菌株Bradyrhizobiumjaponicum和Bradyrhizobiumelkanii相同 .图 1表 2参Two hundred and forty five slow growing rhizobia of Glycine max which came from northern Thailand were analyzed by amplified restriction fragment polymorphism (AFLP) technique. According to the AFLP banding patterns,the results showed that most of these isolates were genetically diversified. Ninety two isolates were selected to represent the diversity of the 245 isolates . The 92 isolates were clustered into eight groups at the level of similarity of 82% by computer analysis of the data. Part of the representative isolates and reference strains of slow growing rhizobia of Glycine max were identified by restriction fragment length polymorphism (RFLP) analysis of PCR Amplified 16S rDNA. Two different 16S rDNA PCR RFLP types were found, which are the same as Bradyrhizobium japonicum and Bradyrhizobium elkanii , respectively. Fig 1, Tab 2, Ref 14

关 键 词：AFLP 慢生型大豆根瘤菌 遗传多样性 

分 类 号：Q939.11[生物学—微生物学]