高效液相色谱法同时测定葛枳胶囊中5种有效成分的含量  

Simultaneous HPLC Determination of 5 Active Components in the TCM of "Gezhi" Capsule

作　　者：潘丽丽[1] 韩孟书[1,2] 江帆[1] 

机构地区：[1]贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室,贵阳550001 [2]贵阳云岩青青生物科技有限公司,贵阳550001

出　　处：《理化检验（化学分册）》2013年第3期290-293,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：贵阳市科学技术计划项目([2009]筑科农8-004号);贵阳市云岩区科学技术计划项目[(2010)18号]

摘　　要：提出了高效液相色谱法同时测定葛枳胶囊中5种有效成分(葛根素、大豆苷、甘草苷、大豆苷元和甘草酸)的含量。样品经甲醇-水(50+50)溶液超声提取30min,采用Diamonsil C18色谱柱(200mm×4.6mm,5μm)为分离柱,以不同体积比混合的乙腈和磷酸(0.1+99.9)混合液为流动相进行梯度洗脱,甘草苷的检测波长为276nm,其余4种成分均为250nm。5种有效成分分别在一定的质量浓度范围内与其峰面积呈线性关系,方法的检出限(3S/N)在0.050～0.338mg·L-1之间。方法的回收率在97.1%～104%之间,测定值的相对标准偏差(n=6)在1.0%～2.7%之间。A method of HPLC for the determination of 5 active components,including puerarin,daidzin,liquiritin,daidzein and glycyrrhizic acid,in the TCM ＂Gezhi＂ capsule was proposed.The sample was extracted with mixture of methanol and H2O（50＋50） for 30 min.The extract was separated on Diamonsil C18 column（200 mm×4.6 mm,5 μm） by gradient elution with mixtures of methanol and dil.phosphoric acid（0.1＋99.9） solution in different ratios,and determined at the wavelength of 250 nm except for liquirtin at 276 nm.Linear relationships between peak area and mass concentration of the 5 active components were kept in the definite ranges,with detection limits（3S/N） in the range of 0.050-0.338 mg·L-1.Values of average recovery of the method found were in the range of 97.1%-104% and RSD′s（n=6） found were in the range of 1.0%-2.7% respectively.

关 键 词：高效液相色谱法 葛枳胶囊 葛根素 大豆苷 甘草苷 大豆苷元 甘草酸 

分 类 号：O652.63[理学—分析化学]