无病毒草莓良种繁育技术体系的建立  

Techniques for propagation of virus-free strawberry

作　　者：刘庆忠[1] 赵红军[1] 姜君军 王茂兴 沈文书 

机构地区：[1]山东省果树研究所,泰安271000 [2]烟台市农业学校 [3]莒南县草莓研究所

出　　处：《落叶果树》2000年第4期1-4,共4页Deciduous Fruits

摘　　要：利用茎尖分生组织 ( 0 .3mm)培养技术 ,在MS附加 1 .3 2 μmol 6-苄基嘌呤的培养基上培养 ,获得了早美光、矮丰、瑞星、奇妙、北斗和全明星 6个草莓品种的无病毒苗。将微体繁殖技术与大田生产相结合 ,建立了 6个草莓品种从无病毒组培“原原种”苗到“生产”用苗的良繁技术体系 ,年内增殖可达数万倍以上 ,比传统的热处理脱毒。Virus-free strawberry stocks were obtained from Earliglow, Mohawk, Allstar, Northeaster, Demarvel and Latestar cultivars by using shoot meristem (0.3mm) culture on the MS medium supplemented with 1.32umol 6-benzyladenine. The propagation technique system from in vitro virus-free “elite stocks” to “production” stocks could get tens of thousands times, more efficient than conventional technique of heat-treatment-eradicating virus and stock propagation in isolation chambers.

关 键 词：无病毒草莓 分生组织培养 微体繁殖 良种繁育 

分 类 号：S668.403.8[农业科学—果树学]