茜素/石墨烯-壳聚糖修饰玻碳电极应用于人类端粒DNA的测定  

Application of Alizarin/Graphene-Chitosan Modified Electrode on Detection of Human Telomere DNA

作　　者：蒋园园[1] 王坤[1] 许崇正[1] 杨小弟[1] 李卉卉[1] 

机构地区：[1]江苏省生物功能材料重点实验室,江苏省新型动力电池重点实验室,南京师范大学化学与材料科学学院,南京210097

出　　处：《分析化学》2013年第4期481-487,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目(Nos.20875047,20902048);水利部专项基金资助项目(No.201201018);江苏高校优势学科建设工程资助项目

摘　　要：采用茜素(AR)掺杂石墨烯(CRG)、壳聚糖(CS)制备了AR/CRG-CS电极。该修饰电极在pH 7.4的PBS中出现了一对可逆的氧化还原峰,阳极峰的峰电位为"0.573 V,阴极峰峰电位为"0.652 V。通过循环伏安法(CV)证明AR在电极上发生了两电子两质子的氧化还原可逆过程,电子传递速率Ks为1.69 s"1。基于AR与人类端粒DNA相互作用导致的AR峰电流减弱及式量电位(E0')负移的电化学信号,建立了一种灵敏简便、重现性好、稳定性好的测定人类端粒DNA的电化学方法,线性响应范围为8×10"8～1.4×10"5mol/L(R=0.9995),线性回归方程为Δi(μA)=8.4860+0.5366C(μmol/L),检出限为2×10"8mol/L(S/N=3)。A modified glassy carbon electrode of alizarin（AR） intermingled with graphene（CRG） and chitosan（CS） was prepared for the first time.The AR/CRG-CS electrode exhibited a pair of well-defined reversible redox peaks in 0.1 mol/L phosphate buffer solution（PBS,pH 7.4） with the oxidation peak potential of 0.573 V and the reduction peak potential of 0.652 V.It exhibited a two-proton and two-electron redox process and a fast heterogeneous electron transfer rate with the rate constant（Ks） of 1.69 s1 using the cyclic voltammogram.Based on the characteristics of alizarin with the decreased current and negative potential after binding with human telomere DNA,a sensitive,simple,reliable and stable electrochemical method for the detection of alizarin using the AR/CRG-CS electrode was developed.The working curve was constructed in the range of 8×10^-8-1.4×10^-5 mol/L（R=0.9995） with the linear regression equation as Δi（μA）=8.4860＋0.5366c（μmol） and the detection limit（S/N） as 2×10^-8 mol/L.

关 键 词：茜素 端粒DNA 相互作用 修饰电极 电化学检测 

分 类 号：R346[医药卫生—基础医学]