光谱法研究恩曲他滨与DNA的相互作用  

Study on Interaction between Emtricitabine and DNA by Spectroscopy Methods

作　　者：孟彦波[1] 

机构地区：[1]邢台医学高等专科学校,河北邢台054000

出　　处：《中国药业》2013年第6期13-14,共2页China Pharmaceuticals

摘　　要：目的研究恩曲他滨与小牛胸腺DNA相互作用的方式。方法采用紫外分光光度法和荧光光谱法。结果恩曲他滨是通过嵌插方式与DNA发生相互作用。结论恩曲他滨与DNA的相互作用为静态淬灭过程,其淬灭速率常数Kq=7.8×1010L/(mol.s),结合常数K=1.01×103L/mol,结合位点数n=1.02。Objective To study the interaction way of emtricitabine and calf thymus（ct） -DNA. Methods The ultraviolet spectrophotom- etry and the fluorescence spectrofluorometric method were adopted. Results Erntricitabine was confirmed to interact with DNA by the intercalation binding mode. Conclusion The interaction of emtricitabine with DNA is static quenching process,the quenching rate con- stant K, =7.8 × 10^10L/（mol · s） ,the binding constant K= 1.01 × 10^3 L/mol,the binding site n = 1.02.

关 键 词：恩曲他滨 小牛胸腺DNA 荧光淬灭 嵌插作用 

分 类 号：R966[医药卫生—药理学] R979.1[医药卫生—药学]