乐至黑山羊与藏山羊HSC70、HSP70.1和HSF1基因的mRNA表达差异分析  

Analysis of mRNA Differential Expression of HSC70、HSP70.1 and HSF1 Gene in Lezhi Black Goat and Tibetan Goat

作　　者：牛惠然[1] 字向东[1] 任陶 王永[1] 任称罗 尔日 朱万岭 付锡三 

机构地区：[1]西南民族大学、青藏高原动物遗传资源保护与利用四川省重点实验室,四川成都610041 [2]四川省理县畜牧局,四川理县623100 [3]四川省乐至县科技局,四川乐至641500

出　　处：《家畜生态学报》2013年第2期11-14,共4页Journal of Domestic Animal Ecology

基　　金：中央高校基本科研业务费优秀学生培养工程项目(12ZYXS71);国家科技支撑计划课题(2008BADC3B01)

摘　　要：试验旨在利用qPCR技术对热休克同源蛋白(HSC70)、热休克蛋白70.1(HSP70.1)和热休克转录因子1(HSF1)基因在乐至黑山羊和藏山羊五个组织样中的表达量进行研究,以探讨热休克蛋白家族70(HSP70)与山羊生态适应性的关系。结果表明,HSC70mRNA在藏山羊心脏中的表达量是乐至黑山羊中的4.5倍(P<0.05),但在脑垂体中乐至黑山羊的表达量是藏山羊的3.7倍(P<0.05),在卵巢、肝脏和子宫中两品种间无显著差异;HSF1mRNA在藏山羊卵巢、心脏中的表达量分别是乐至黑山羊的7.9倍和3.1倍(P<0.05),在肝脏和子宫中乐至黑山羊的表达量却分别是藏山羊的6.3倍和10.4倍(P<0.05);HSP70.1mRNA在乐至黑山羊脑垂体中的表达量是藏山羊的6.8倍(P<0.05)。说明HSC70、HSP70.1和HSF1基因的表达与山羊的生活环境及饲养方式有一定的关系。In order to investigate the relationship between eco adaptation and heat shock protein70, the heat shock cognate protein 70 （HSC70）, heat shock protein 70.1 （HSPT0. 1） and the heat shock tran scription factor 1 （HSF1） genes in five tissue samples of Lezhi black goat and Tibetan goat were deter mined by qPCR. The results showed that the expression of HSC70 mRNA in Tibetan goat＇s heart was 4.5 times to that of the Lezhi black goat （P〈0.05）, but in pituitary, Lezhi black goat is 3.7 times to Tibetan goat. Its expression in the ovary, liver, uterus, was not different between the two breeds. The expression of HSF1 mRNA in Tibetan goat ovarian, heart was 7.9, 3.1 times to that of the Lezhi black goat （P〈0.05）, however, in the liver and uterus tissue samples, Lezhi black goat was in high expression levels than Tibetan goat （6.3 fold, 10.4 fold; P〈0.05）. The expression of HSPT0.1 mRNA in Lezhi black goat pi tuitary was 6.8 times to Tibetan goat （P〈0.05）. It could be concluded that the difference in the expres sion of HSC70, HSPTO. 1 and HSF1 wight be related to the eco-environment and rearing methods （graz- ing） in Lezhi black goat and Tibetan goat.

关 键 词：山羊 qPCR HSC70基因 HSPTO 1基因 HSF1基因 

分 类 号：S811.6[农业科学—畜牧学]