攀西黑山羊群体的AFLP遗传多样性研究  

Study on Genetic Diversity Based on AFLP Markers on Black Goat in the West of Panzhihua

作　　者：李娜[1] 杨鹰[2] 万洁[2] 韦雷飞 钟金城[1] 武志娟[1] 姜雪鸥[1] 张利亚[1] 

机构地区：[1]动物遗传育种学国家民委-教育部重点实验室,西南民族大学,四川成都610041 [2]攀枝花市农林科学研究院,四川攀枝花617061

出　　处：《家畜生态学报》2013年第2期22-26,68,共6页Journal of Domestic Animal Ecology

基　　金：攀枝花市科技计划项目(2010CY-N-1)

摘　　要：研究采用随机片段长度多态性分析了攀西地区的5个黑山羊品种(类群)的遗传多样性。结果表明:15对引物共扩增出1003条多态条带,多态频率平均为99.01%,5个黑山羊群体的遗传多样性指数变异范围为0.0136～0.2131,其中建昌黑山羊平均遗传多样性指数最高(0.1346),攀枝花黑山羊次之(0.1329),乐至黑山羊最低(0.1101)。5个群体遗传距离范围为0.0062～0.0281,攀枝花黑山羊与建昌黑山羊之间的遗传距离最小(DR=0.0062),云岭黑山羊与攀枝花黑山羊之间的遗传距离次之(DR=0.0128),乐至黑山羊与金堂黑山羊间遗传距离最大(DR=0.0281),金堂黑山羊与其它4个类群遗传距离较大(DR=0.0173-0.0281),说明攀枝花黑山羊与建昌黑山羊的亲缘关系最近,与云岭黑山羊的亲缘关系较近,乐至黑山羊与金堂黑山羊的亲缘关系最远,金堂黑山羊与其它4个类群遗传距离均比较远。聚类结果与群体地理分布、生态条件差异情况基本一致。In this study, AFLP（Amplified Fragment Length Polymorphism） was employed to analyze genetic diversity of 5 black goat populations in Panzhihua. 15 primer combinations were used to amplify 1003 polymorphic markers, the average polymorphic frequency is 99.01%. The genetic diversity indexes of the 5 black goat populations are from 0. 0136 to 0. 2131, the maximum and minimum average genetic diver- sity indexes are Jianchang（0. 1346）and Lezhi（0. 1101） respectively. The genetic distance is from 0. 0062 to 0. 0281. Aa a result, Jianchang and Panzhihua black goats are clustered into a class, Yunling and Lezhi black goats are clustered into a class, the above two classes are clustered into a large one~ the large class is clustered with Jingtang black goat into one class. The of AFLP could reflect the influence of environment conditions on genetic diversity.

关 键 词：黑山羊 AFLP 遗传多样性 

分 类 号：S811.6[农业科学—畜牧学]