用异源蛋白和蛋壳体外培养鹌鹑胚胎的研究  

Study on the Quail Embryo Culture In Vitro by Foreign Albumen and Eggshell

作　　者：张文昌[1] 李玉芳[2] 庄益芬[1] 

机构地区：[1]福建农林大学动物科学学院,福建福州350002 [2]黄淮学院,河南驻马店463000

出　　处：《家畜生态学报》2013年第2期38-40,共3页Journal of Domestic Animal Ecology

基　　金：福建省自然科学基金项目(2007J0042)

摘　　要：将50个鹌鹑胚胎在鸡蛋壳中用鸡稀蛋白培养,对种蛋产出体外阶段裸黄状态的鹌鹑胚胎进行培养的方法进行了探讨。1～2.5d、2.5～14d和15～17d胚胎培养温度分别是38.0℃、37.8℃和37.5℃,相对湿度分别是60%、55%和70%,1～14d每小时翻蛋1次,翻蛋角度1～2.5d为90°,2.5～14d为50°,15d后静止落盘。2.5d和15d胚胎存活率以及孵化率分别为92%、78%和28%。结果表明裸黄状态的鹌鹑胚胎不排斥鸡稀蛋白,用鸡稀蛋白培养鹌鹑胚胎可行。Fifty quail embryos with egg yolk but without albumen were used to examine the possibility of its culture by thin albumen from chicken eggs in the chicken eggshell. The quail embryos were cultured in an incubator at 38.0 ℃ and 60% for RH from day 1 to 2.5, 37.8 ℃ and 55% for RH from days 2.5 to 14, 37.5 ℃ and 70%for RH from days 15 to 17. The chicken eggshells with quail embryos were rocked at an angle of 90 for the first 2.5 days and 50 from day 2.5 to 14, once every hour. Stopped turning after 14 days. The survival rate of the quail embryos was 92℃ at day 2.5, 78% at day 15. The hatchability was 28%. The result suggested that thin albumen from chicken eggs was not rejected by the quail embryo, and it was a good in vitro culture medium for the quail embryo.

关 键 词：鹌鹑 胚胎 培养 鸡稀蛋白 鸡蛋壳 

分 类 号：S811.6[农业科学—畜牧学]