去卵巢大鼠骨计量学参数和血脂的变化及意义  

Changes of bone histomorphometry parameters and serum lipid in rats after ovariectomized 30 days

作　　者：尹斯利[1] 金勋杰[2] 胡彬[2] 黄连芳[2] 李青南[2] 

机构地区：[1]广东医学院附属医院检验科,524023 [2]广东医学院骨生物学研究室

出　　处：《中国骨质疏松杂志》2000年第2期42-43,86,共3页Chinese Journal of Osteoporosis

基　　金：广东省卫生厅"五个一科教兴医工程"基金

摘　　要：目的　观察大鼠去卵巢 30 d时骨量、骨结构及血脂的变化。方法　 17只 SD雌性大鼠随机分为 2组 :假手术组、去卵巢组。实验 30 d,处死大鼠取胫骨及血清 ,用骨计量学方法观察骨量和骨结构变化 ,并检测血清碱性磷酸酶 (AKP)、钙 (Ca)、磷 (P)、总胆固醇 (CH)、甘油三脂 (TG)。结果　去卵巢组与假手术组比 ,骨小梁面积和数量减少 (P<0 .0 1) ,骨小梁间隙增加 (P<0 .0 5) ;血液检验显示 AKP增加 (P<0 .0 1) ,CH增加 (P<0 .0 5) ,TG降低 (P<0 .0 1)。结论　本文结果显示 30 d去卵巢大鼠已出现典型的骨质疏松表现 ,伴有血脂的紊乱。Objective To study the changes of rats bone volume and serum lipid on ovariectomized rats.Methods 17 female SD inbred rats were divided into 2 groups:sham ovx group and ovx group,30 days experiment period,the proximal tibial metaphysis(PTM)were processed undecalcified for quantitative bone histomorphometry and serum were collected to measure alkaline phosphatase(AKP),calcium(Ca),phosphorus(P),cholesterol(CH)and triglyceride(TG).Results Trabecular area percent(Tb.Ar%)and Trabecular number(Tb.N)of ovx rats decreased ( P <0.01),trabecular separation(Tb.Sp)increased( P <0.05)compared with sham ovx group.Compared with sham ovx group,the AKP level of ovx group increased ( P <0 01),Ca P has no change,CH increased ( P <0.05),TG decreased( <0.01).Conclusion The data suggests that 30 days after ovariectomy,rats'bone volume decreased,while serum CH level increased and TG level decreased.

关 键 词：骨质疏松 骨组织形态计量学 总胆固醇 甘油三脂 

分 类 号：R681[医药卫生—骨科学]